Detailed information of TA system TA058282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 312541..313156
• Replicon: chromosome
• Accession: NZ_CP013308
• Organism: Vibrio cholerae strain E506

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ84_RS15380
• Protein ID: WP_001162670.1
• Coordinates: 312541..312828 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ84_RS15385
• Protein ID: WP_001232701.1
• Coordinates: 312839..313156 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ84_RS15330	307842..308393	+	552	WP_001894072.1	translation initiation factor IF-3	-
ASZ84_RS15335	308497..308691	+	195	WP_001885060.1	50S ribosomal protein L35	-
ASZ84_RS15340	308733..309086	+	354	WP_001138366.1	50S ribosomal protein L20	-
ASZ84_RS15345	309168..310130	-	963	WP_000841995.1	integron integrase IntIA	-
ASZ84_RS15350	310920..311177	+	258	WP_080379067.1	hypothetical protein	-
ASZ84_RS15355	311360..311773	+	414	WP_000049417.1	VOC family protein	-
ASZ84_RS15370	312024..312260	+	237	WP_000868090.1	DUF645 family protein	-
ASZ84_RS15380	312541..312828	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ84_RS15385	312839..313156	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ84_RS20400	313153..313263	+	111	WP_172837665.1	DUF3265 domain-containing protein	-
ASZ84_RS20405	313496..313591	+	96	WP_001907607.1	DUF645 family protein	-
ASZ84_RS15395	313986..314570	+	585	WP_197668439.1	ATP-binding protein	-
ASZ84_RS15405	314866..315045	+	180	WP_016711064.1	DUF645 family protein	-
ASZ84_RS15410	315287..315517	-	231	WP_000838533.1	PAS factor family protein	-
ASZ84_RS15415	315741..316598	+	858	WP_001894914.1	DUF2806 domain-containing protein	-
ASZ84_RS15425	316745..317644	+	900	WP_001887493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304088..451655	147567
	-	inside	Integron	-	-	309168..451279	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58282 WP_001162670.1 NZ_CP013308:312541-312828 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58282 NZ_CP013308:312541-312828 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58282 WP_001232701.1 NZ_CP013308:312839-313156 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58282 NZ_CP013308:312839-313156 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36