Detailed information of TA system TA058246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 381898..382513
• Replicon: chromosome
• Accession: NZ_CP013304
• Organism: Vibrio cholerae strain CRC711

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: ASZ82_RS16270
• Protein ID: WP_001162670.1
• Coordinates: 381898..382185 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ASZ82_RS16275
• Protein ID: WP_001232701.1
• Coordinates: 382196..382513 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ82_RS20445	377534..377629	+	96	WP_001907607.1	DUF645 family protein	-
ASZ82_RS16230	377823..378140	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ82_RS16235	378159..378401	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ82_RS16240	378643..379152	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
ASZ82_RS16245	379209..379862	+	654	WP_000226874.1	hypothetical protein	-
ASZ82_RS16250	380172..380390	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
ASZ82_RS16260	380793..381026	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
ASZ82_RS16265	381451..381631	+	181	Protein_381	DUF645 family protein	-
ASZ82_RS16270	381898..382185	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASZ82_RS16275	382196..382513	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
ASZ82_RS20450	382510..382620	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
ASZ82_RS20455	382797..382922	+	126	WP_001944767.1	DUF645 family protein	-
ASZ82_RS20460	382938..382976	+	39	WP_106019119.1	hypothetical protein	-
ASZ82_RS16295	383200..383940	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
ASZ82_RS16300	384145..384975	+	831	WP_000056616.1	abortive infection family protein	-
ASZ82_RS16305	385032..385514	+	483	WP_001895003.1	hypothetical protein	-
ASZ82_RS16315	385961..386836	+	876	WP_000259044.1	hypothetical protein	-
ASZ82_RS16320	386966..387421	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	304736..427674	122938
	-	inside	Integron	-	-	309816..427298	117482

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T58246 WP_001162670.1 NZ_CP013304:381898-382185 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T58246 NZ_CP013304:381898-382185 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT58246 WP_001232701.1 NZ_CP013304:382196-382513 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT58246 NZ_CP013304:382196-382513 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36