Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3252988..3253583 Replicon chromosome
Accession NZ_CP013245
Organism Pseudomonas aeruginosa strain VA-134

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag ATC05_RS29760 Protein ID WP_003113526.1
Coordinates 3252988..3253266 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag ATC05_RS15000 Protein ID WP_003111575.1
Coordinates 3253278..3253583 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ATC05_RS14985 3248414..3249652 + 1239 WP_003113524.1 dipeptidase -
ATC05_RS14990 3249714..3250361 + 648 WP_003095021.1 carbonate dehydratase -
ATC05_RS14995 3250431..3252659 - 2229 WP_003457077.1 TonB-dependent receptor -
ATC05_RS29760 3252988..3253266 + 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ATC05_RS15000 3253278..3253583 + 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
ATC05_RS15010 3253990..3255090 - 1101 WP_003099270.1 redox-regulated ATPase YchF -
ATC05_RS15015 3255131..3255715 - 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
ATC05_RS15020 3255758..3256372 - 615 WP_003099279.1 50S ribosomal protein L25/general stress protein Ctc -
ATC05_RS15025 3256489..3257430 - 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
ATC05_RS15035 3257597..3258445 - 849 WP_003117426.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T58189 WP_003113526.1 NZ_CP013245:3252988-3253266 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T58189 NZ_CP013245:3252988-3253266 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT58189 WP_003111575.1 NZ_CP013245:3253278-3253583 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT58189 NZ_CP013245:3253278-3253583 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References