Detailed information of TA system TA058182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5578361..5579002
• Replicon: chromosome
• Accession: NZ_CP013236
• Organism: Collimonas pratensis strain Ter291

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CPter291_RS25640
• Protein ID: WP_082807362.1
• Coordinates: 5578361..5578681 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A127R595
• Locus tag: CPter291_RS25090
• Protein ID: WP_062120153.1
• Coordinates: 5578691..5579002 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CPter291_RS25055	5573691..5574266	+	576	WP_062120139.1	DUF1211 domain-containing protein	-
CPter291_RS25060	5574315..5574650	+	336	WP_062120142.1	hypothetical protein	-
CPter291_RS25065	5574891..5575349	-	459	WP_062120145.1	hypothetical protein	-
CPter291_RS25070	5575352..5575636	-	285	WP_062120147.1	DUF4258 domain-containing protein	-
CPter291_RS25075	5575834..5576463	+	630	WP_156480181.1	hypothetical protein	-
CPter291_RS25080	5576481..5577563	+	1083	WP_061945637.1	AI-2E family transporter	-
CPter291_RS25085	5577585..5578193	-	609	WP_062120150.1	DUF1294 domain-containing protein	-
CPter291_RS25640	5578361..5578681	+	321	WP_082807362.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CPter291_RS25090	5578691..5579002	+	312	WP_062120153.1	HigA family addiction module antidote protein	Antitoxin
CPter291_RS25095	5579099..5579830	-	732	WP_062120156.1	hypothetical protein	-
CPter291_RS26255	5580056..5580334	-	279	WP_156480182.1	hypothetical protein	-
CPter291_RS25100	5580472..5581074	-	603	WP_062120159.1	hypothetical protein	-
CPter291_RS25105	5581241..5582638	-	1398	WP_062120162.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (22-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12002.80 Da
• Isoelectric Point: 10.7341

>T58182 WP_082807362.1 NZ_CP013236:5578361-5578681 [Collimonas pratensis]
MQRDVPAGFSRRVVIIGLIYETDTRALYQGTRVAKFVNIHAVAERKLQLLDSAATLNFLRSPPGNRLQALVGNRAGQHSI
RINDQWRICFVWTGNGVKNVEIVDYH

Nucleotide

• Length: 321 bp

>T58182 NZ_CP013236:5578361-5578681 [Collimonas pratensis]
ATGCAAAGAGATGTTCCTGCTGGCTTCTCCAGGCGTGTGGTCATCATTGGTTTGATTTATGAGACAGACACACGAGCCTT
GTATCAGGGGACGCGAGTTGCAAAGTTTGTAAATATTCATGCTGTCGCAGAGCGCAAGCTTCAATTGCTTGATAGCGCCG
CAACGCTGAATTTCCTGCGCTCGCCACCGGGTAATCGTTTGCAGGCTTTAGTTGGAAATCGCGCCGGACAACACAGTATC
CGGATTAACGATCAGTGGCGCATTTGCTTCGTCTGGACTGGCAATGGCGTAAAAAACGTCGAAATCGTTGATTACCACTA
G

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11546.40 Da
• Isoelectric Point: 8.7220

>AT58182 WP_062120153.1 NZ_CP013236:5578691-5579002 [Collimonas pratensis]
MSKIQNRMRPIHPGEILREEFLLPSNMSANALAVALNVPATRIGEILHGHRSITPDTALRLARYFGGDASSWLNLQQSFD
LKIAEQEILPRITKEVKPMAETI

Nucleotide

• Length: 312 bp

>AT58182 NZ_CP013236:5578691-5579002 [Collimonas pratensis]
ATGAGCAAAATTCAAAACCGGATGCGTCCGATTCACCCGGGAGAAATTCTCAGGGAAGAGTTTTTGCTGCCATCGAACAT
GAGCGCGAATGCCCTGGCCGTAGCCCTGAACGTCCCCGCAACCAGGATAGGCGAAATCCTCCATGGACACCGCAGCATCA
CACCAGATACGGCGTTGCGCCTGGCACGTTATTTTGGAGGAGATGCATCGTCCTGGTTGAATCTCCAGCAAAGCTTTGAT
CTGAAAATAGCTGAGCAGGAAATTCTTCCGAGGATTACCAAAGAGGTCAAACCGATGGCAGAGACAATATAG

Structures

Antitoxin

• AlphaFold DB: A0A127R595