Detailed information of TA system TA058106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3739560..3740254
• Replicon: chromosome
• Accession: NZ_CP013190
• Organism: Escherichia coli strain FORC_031

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FORC31_RS18650
• Protein ID: WP_001263493.1
• Coordinates: 3739560..3739958 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FORC31_RS18655
• Protein ID: WP_000554757.1
• Coordinates: 3739961..3740254 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC31_RS18620	3734560..3735804	-	1245	WP_000189541.1	esterase FrsA	-
-	3735220..3735300	-	81	NuclAT_12	-	-
-	3735220..3735300	-	81	NuclAT_12	-	-
-	3735220..3735300	-	81	NuclAT_12	-	-
-	3735220..3735300	-	81	NuclAT_12	-	-
FORC31_RS18625	3735896..3736354	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FORC31_RS18630	3736615..3738072	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FORC31_RS18635	3738129..3738650	-	522	Protein_3579	peptide chain release factor H	-
FORC31_RS18640	3738649..3738852	-	204	Protein_3580	RNA ligase RtcB family protein	-
FORC31_RS18645	3739098..3739550	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FORC31_RS18650	3739560..3739958	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FORC31_RS18655	3739961..3740254	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FORC31_RS18660	3740306..3741361	-	1056	WP_044316732.1	DNA polymerase IV	-
FORC31_RS18665	3741432..3742355	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
FORC31_RS18670	3742358..3743221	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
FORC31_RS18675	3743234..3743953	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
FORC31_RS18680	3743973..3744440	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T58106 WP_001263493.1 NZ_CP013190:c3739958-3739560 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T58106 NZ_CP013190:c3739958-3739560 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT58106 WP_000554757.1 NZ_CP013190:c3740254-3739961 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT58106 NZ_CP013190:c3740254-3739961 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6