Detailed information of TA system TA058081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 235590..236165
• Replicon: chromosome
• Accession: NZ_CP013189
• Organism: Pseudohongiella spirulinae strain KCTC 32221

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PS2015_RS01185
• Protein ID: WP_058020449.1
• Coordinates: 235590..235868 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: A0A0S2K9D0
• Locus tag: PS2015_RS01190
• Protein ID: WP_058020450.1
• Coordinates: 235878..236165 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PS2015_RS01125	230704..231237	+	534	WP_058023077.1	GrpB family protein	-
PS2015_RS01130	231372..231947	+	576	WP_058020439.1	hypothetical protein	-
PS2015_RS01135	232073..232531	+	459	WP_058020440.1	hypothetical protein	-
PS2015_RS01145	232720..233001	+	282	WP_058020442.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
PS2015_RS01150	233001..233300	+	300	WP_058023078.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PS2015_RS01160	233690..234028	+	339	WP_058020444.1	toxin-antitoxin system HicB family antitoxin	-
PS2015_RS01165	234080..234304	-	225	WP_058020445.1	CopG family transcriptional regulator	-
PS2015_RS01170	234309..234731	-	423	WP_058020446.1	putative toxin-antitoxin system toxin component, PIN family	-
PS2015_RS01175	234922..235200	+	279	WP_058020447.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
PS2015_RS01180	235204..235464	+	261	WP_058020448.1	helix-turn-helix domain-containing protein	-
PS2015_RS01185	235590..235868	+	279	WP_058020449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PS2015_RS01190	235878..236165	+	288	WP_058020450.1	HigA family addiction module antidote protein	Antitoxin
PS2015_RS01195	236311..236568	+	258	WP_058020451.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
PS2015_RS01200	236556..236867	+	312	WP_058023079.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PS2015_RS01205	237007..237321	+	315	WP_058020452.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PS2015_RS01210	237308..237586	+	279	WP_058020453.1	putative addiction module antidote protein	-
PS2015_RS01215	237735..237998	+	264	WP_058020454.1	BrnT family toxin	-
PS2015_RS01220	237988..238257	+	270	WP_058020455.1	hypothetical protein	-
PS2015_RS01225	238326..238628	+	303	WP_058020456.1	type II toxin-antitoxin system HigB family toxin	-
PS2015_RS01230	238637..239017	+	381	WP_058020457.1	hypothetical protein	-
PS2015_RS01235	239141..239383	+	243	WP_058023080.1	hypothetical protein	-
PS2015_RS01240	239428..239706	+	279	WP_058020458.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PS2015_RS01245	239706..239999	+	294	WP_058020459.1	HigA family addiction module antidote protein	-
PS2015_RS01250	240073..240465	-	393	WP_058020460.1	PIN domain nuclease	-
PS2015_RS01255	240462..240656	-	195	WP_058020461.1	type II toxin-antitoxin system VapB family antitoxin	-
PS2015_RS01260	240839..241093	+	255	WP_058020462.1	type II toxin-antitoxin system HicA family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	226994..240465	13471
	-	inside	Prophage	-	-	237007..239999	2992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10327.80 Da
• Isoelectric Point: 10.3761

>T58081 WP_058020449.1 NZ_CP013189:235590-235868 [Pseudohongiella spirulinae]
MIRSFKHKGLAKFFKSGSTAGIQADHAKRLRLILGRLHAAASPKDMDLPGLRLHELTGNRSGTWSVMVSGNWRVTFVFQG
EDAEVVNYEDYH

Nucleotide

• Length: 279 bp

>T58081 NZ_CP013189:235590-235868 [Pseudohongiella spirulinae]
ATGATTCGGAGCTTCAAACACAAGGGATTGGCCAAATTCTTCAAATCCGGCAGTACTGCTGGGATTCAGGCCGATCATGC
AAAGCGGCTCCGGCTAATCCTCGGTCGTCTTCATGCAGCCGCGTCACCAAAGGATATGGATCTGCCAGGGCTTCGGCTTC
ATGAGCTCACTGGCAACCGTTCAGGTACTTGGTCAGTAATGGTAAGCGGTAACTGGCGAGTCACATTTGTTTTTCAAGGT
GAAGATGCTGAGGTCGTCAACTATGAAGACTATCATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10385.93 Da
• Isoelectric Point: 6.4756

>AT58081 WP_058020450.1 NZ_CP013189:235878-236165 [Pseudohongiella spirulinae]
MLMHNPPHPGEVIKELCLQPLGISVTDAAEALGVSRKTLSAILNGRAGISPEMAVRLSIAFDTSAESWLNQQSQYELWHA
EQHRGELQVKKLVAA

Nucleotide

• Length: 288 bp

>AT58081 NZ_CP013189:235878-236165 [Pseudohongiella spirulinae]
ATGTTAATGCACAATCCCCCTCATCCAGGCGAGGTCATTAAAGAGCTGTGCCTTCAGCCTTTAGGCATCTCAGTGACGGA
TGCGGCCGAGGCACTCGGTGTCAGCAGGAAGACTCTAAGCGCAATTCTCAACGGCCGCGCCGGAATCAGTCCGGAAATGG
CTGTACGGCTCTCCATCGCATTCGACACGTCAGCTGAAAGCTGGCTCAACCAACAGTCCCAATACGAGCTATGGCATGCC
GAGCAACATAGAGGCGAGCTTCAGGTTAAGAAATTGGTTGCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S2K9D0