Detailed information of TA system TA058074

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5925..6351
• Replicon: plasmid FORC_029p
• Accession: NZ_CP013186
• Organism: Escherichia coli strain FORC_029

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FORC29_RS27230
• Protein ID: WP_001388592.1
• Coordinates: 5925..6083 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	6127..6351 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC29_RS27190	1313..1540	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
FORC29_RS27195	1628..2320	-	693	WP_000332477.1	PAS domain-containing protein	-
FORC29_RS27200	2514..2897	-	384	WP_001151566.1	relaxosome protein TraM	-
FORC29_RS27205	3237..3827	+	591	WP_176453891.1	transglycosylase SLT domain-containing protein	-
FORC29_RS27215	4122..4943	-	822	WP_001234482.1	DUF945 domain-containing protein	-
FORC29_RS28060	5122..5687	+	566	Protein_9	hypothetical protein	-
FORC29_RS27230	5925..6083	-	159	WP_001388592.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	6127..6351	-	225	NuclAT_0	-	Antitoxin
-	6127..6351	-	225	NuclAT_0	-	Antitoxin
-	6127..6351	-	225	NuclAT_0	-	Antitoxin
-	6127..6351	-	225	NuclAT_0	-	Antitoxin
FORC29_RS27235	6163..6342	+	180	WP_001309233.1	hypothetical protein	-
FORC29_RS27240	6363..6965	-	603	Protein_12	plasmid SOS inhibition protein A	-
FORC29_RS27260	8065..9732	+	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
FORC29_RS28275	9869..9985	+	117	Protein_14	pilus protein	-
FORC29_RS27270	10071..10253	-	183	Protein_15	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	pic / aap/aspU / agg3A / agg3B / agg3C / agg3D	1..77339	77339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6135.36 Da
• Isoelectric Point: 10.1101

>T58074 WP_001388592.1 NZ_CP013186:c6083-5925 [Escherichia coli]
MKLPRSSLVWRVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T58074 NZ_CP013186:c6083-5925 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGCGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT58074 NZ_CP013186:c6351-6127 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTGTATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin