Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-MqsA
Location 1994989..1995622 Replicon chromosome
Accession NZ_CP013145
Organism Colwellia sp. MT41

Toxin (Protein)


Gene name higB Uniprot ID A0A0S2JFW6
Locus tag CMT41_RS08525 Protein ID WP_057183197.1
Coordinates 1994989..1995321 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z5I4P3
Locus tag CMT41_RS08530 Protein ID WP_057183175.1
Coordinates 1995308..1995622 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CMT41_RS08500 1990551..1990952 + 402 WP_057180898.1 fluoride efflux transporter CrcB -
CMT41_RS08505 1991067..1992347 + 1281 WP_057180899.1 serine--tRNA ligase -
CMT41_RS08510 1992753..1993721 - 969 WP_058027020.1 integron integrase -
CMT41_RS08515 1994021..1994479 + 459 WP_058027021.1 hypothetical protein -
CMT41_RS08520 1994610..1994840 + 231 WP_058027022.1 hypothetical protein -
CMT41_RS08525 1994989..1995321 + 333 WP_057183197.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CMT41_RS08530 1995308..1995622 + 315 WP_057183175.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
CMT41_RS08535 1996375..1996731 + 357 WP_058027023.1 hypothetical protein -
CMT41_RS08540 1997050..1998114 - 1065 WP_058027024.1 IS91 family transposase -
CMT41_RS08545 1998107..1999018 - 912 WP_058027025.1 site-specific integrase -
CMT41_RS08550 1999514..2000263 + 750 WP_058027026.1 FRG domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1994506..2001354 6848
- inside Integron - - 1992753..2002948 10195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(8-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(43-92)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12904.99 Da        Isoelectric Point: 10.2228

>T58017 WP_057183197.1 NZ_CP013145:1994989-1995321 [Colwellia sp. MT41]
MKCIFVESKIFEKYRDDHLSDEEFRLFQAELMSNPKKGDVIQGTGGLRKVRVASKGKGKRGGSRVIYYFLDKKRRCYLLP
IYGKSEMSDLTADQKKQLKVFMEVWRNEQS

Download         Length: 333 bp

>T58017 NZ_CP013145:1994989-1995321 [Colwellia sp. MT41]
ATGAAATGTATATTTGTAGAATCAAAAATATTTGAGAAATACCGAGATGACCACCTCAGTGATGAAGAGTTTAGACTGTT
TCAAGCTGAGCTAATGTCAAACCCGAAGAAGGGTGATGTGATTCAAGGTACTGGTGGTTTGCGGAAGGTTCGAGTTGCAA
GTAAGGGTAAAGGAAAACGTGGTGGTTCCCGTGTTATTTATTACTTCCTTGATAAAAAGCGACGTTGCTATTTACTTCCT
ATTTATGGAAAAAGTGAAATGTCGGATTTAACCGCAGATCAAAAGAAACAGTTAAAGGTTTTTATGGAGGTATGGCGTAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11738.23 Da        Isoelectric Point: 6.6066

>AT58017 WP_057183175.1 NZ_CP013145:1995308-1995622 [Colwellia sp. MT41]
MSNRDLFTELSSALVEAKEHSEGKITLKTHHVNDVSELDISPNEIVHIREKFNMSRGVFASLLHTSSRTLENWEQGRSAP
NGQAITLLKLVQRHPETLTHIAEL

Download         Length: 315 bp

>AT58017 NZ_CP013145:1995308-1995622 [Colwellia sp. MT41]
ATGAGCAATCGTGATTTATTTACAGAATTAAGTTCCGCTCTTGTTGAAGCAAAAGAACATTCAGAGGGGAAAATCACCCT
TAAAACGCATCATGTCAACGATGTAAGTGAACTTGATATTTCACCTAATGAAATTGTACATATTAGAGAAAAATTTAATA
TGTCACGTGGTGTGTTTGCTAGTTTGCTTCATACATCTTCTCGTACGTTAGAGAACTGGGAGCAAGGACGCAGTGCTCCA
AATGGGCAAGCAATAACACTTTTGAAATTAGTGCAACGTCATCCAGAAACTCTCACGCACATTGCAGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S2JFW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z5I4P3

References