Detailed information of TA system TA057946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5464364..5464959
• Replicon: chromosome
• Accession: NZ_CP013113
• Organism: Pseudomonas aeruginosa strain Paer4_119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AQ622_RS25965
• Protein ID: WP_071538299.1
• Coordinates: 5464681..5464959 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AQ622_RS25960
• Protein ID: WP_003123432.1
• Coordinates: 5464364..5464669 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQ622_RS25945	5459384..5462380	-	2997	WP_039843332.1	ATP-binding protein	-
AQ622_RS25950	5462397..5462843	-	447	WP_052271067.1	DNA mismatch endonuclease Vsr	-
AQ622_RS25955	5462850..5463875	-	1026	WP_052271065.1	DNA cytosine methyltransferase	-
AQ622_RS25960	5464364..5464669	-	306	WP_003123432.1	HigA family addiction module antidote protein	Antitoxin
AQ622_RS25965	5464681..5464959	-	279	WP_071538299.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQ622_RS25975	5465288..5467516	+	2229	WP_003113525.1	TonB-dependent receptor	-
AQ622_RS25980	5467586..5468233	-	648	WP_003095021.1	carbonate dehydratase	-
AQ622_RS25985	5468295..5469533	-	1239	WP_003123433.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10616.17 Da
• Isoelectric Point: 7.8937

>T57946 WP_071538299.1 NZ_CP013113:c5464959-5464681 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHGIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T57946 NZ_CP013113:c5464959-5464681 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATGGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11204.78 Da
• Isoelectric Point: 4.7769

>AT57946 WP_003123432.1 NZ_CP013113:c5464669-5464364 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT57946 NZ_CP013113:c5464669-5464364 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG