Detailed information of TA system TA057840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4878571..4879301
• Replicon: chromosome
• Accession: NZ_CP013046
• Organism: Serratia marcescens strain B3R3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0P0QJB7
• Locus tag: AR325_RS23380
• Protein ID: WP_060560355.1
• Coordinates: 4878571..4878882 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AR325_RS23385
• Protein ID: WP_033632013.1
• Coordinates: 4878879..4879301 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AR325_RS23350	4873898..4874623	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
AR325_RS23355	4874676..4876109	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
AR325_RS23360	4876133..4876420	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
AR325_RS23365	4876576..4877037	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
AR325_RS23370	4877165..4878019	+	855	WP_015379177.1	RNase adapter RapZ	-
AR325_RS23375	4878016..4878288	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
AR325_RS23380	4878571..4878882	+	312	WP_060560355.1	type II toxin-antitoxin system HigB family toxin	Toxin
AR325_RS23385	4878879..4879301	+	423	WP_033632013.1	helix-turn-helix domain-containing protein	Antitoxin
AR325_RS23390	4879305..4881221	-	1917	WP_015379179.1	BglG family transcription antiterminator	-
AR325_RS23395	4881273..4882487	-	1215	WP_033632011.1	lactonase family protein	-
AR325_RS23400	4882575..4883315	-	741	WP_033648993.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12331.22 Da
• Isoelectric Point: 10.0957

>T57840 WP_060560355.1 NZ_CP013046:4878571-4878882 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRVEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T57840 NZ_CP013046:4878571-4878882 [Serratia marcescens]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAACAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGGGTCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15833.20 Da
• Isoelectric Point: 5.2297

>AT57840 WP_033632013.1 NZ_CP013046:4878879-4879301 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRITQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT57840 NZ_CP013046:4878879-4879301 [Serratia marcescens]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTCGAATTGGTGGAATACCTGCTGGAAAACGATGAACGCCATCCGCTGATTGACATGTTGGCAAAAA
AAATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATAACCCAACTCCCGCAGGGGGTGGCC
GCGCTTCGCGTACTGATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A0P0QJB7