Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4822519..4823147 Replicon chromosome
Accession NZ_CP013046
Organism Serratia marcescens strain B3R3

Toxin (Protein)


Gene name higB Uniprot ID A0A0N7JQB8
Locus tag AR325_RS23070 Protein ID WP_060560346.1
Coordinates 4822519..4822809 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag AR325_RS23075 Protein ID WP_015379150.1
Coordinates 4822824..4823147 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AR325_RS23060 4818933..4820954 + 2022 WP_033632027.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
AR325_RS23065 4821149..4822288 - 1140 WP_049189088.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
AR325_RS23070 4822519..4822809 + 291 WP_060560346.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AR325_RS23075 4822824..4823147 + 324 WP_015379150.1 HigA family addiction module antidote protein Antitoxin
AR325_RS23080 4823140..4823652 + 513 WP_042785417.1 M48 family metallopeptidase -
AR325_RS23085 4823702..4824682 + 981 WP_060560347.1 Gfo/Idh/MocA family oxidoreductase -
AR325_RS23090 4824684..4825415 + 732 WP_060560348.1 glutamine amidotransferase -
AR325_RS23095 4825671..4826636 + 966 WP_167333738.1 TerC family protein -
AR325_RS23100 4826899..4828134 + 1236 WP_004937146.1 serine/threonine transporter SstT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11596.29 Da        Isoelectric Point: 10.2325

>T57839 WP_060560346.1 NZ_CP013046:4822519-4822809 [Serratia marcescens]
MIKSFRDRYLEPFYLEGKRSRLIPGVLERQLARKLDILAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Download         Length: 291 bp

>T57839 NZ_CP013046:4822519-4822809 [Serratia marcescens]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCCGTTCTATCTGGAAGGCAAACGCAGCCGTCTGATCCCCGGCGTGCT
CGAGCGGCAGTTGGCCAGAAAGCTCGATATATTGGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAGCGGCTATCCGGGCCATTTCAGGGATGGTCAAGTCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT57839 WP_015379150.1 NZ_CP013046:4822824-4823147 [Serratia marcescens]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT57839 NZ_CP013046:4822824-4823147 [Serratia marcescens]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGGGAAGTGCTGCAGGAAGAGTATTTAACGCCGTTGGGCATCGG
CCAGGAAGAACTGGCGAAGGTACTGGGCGTCACCCGTGCCACTGTTAACCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAACCTGTTTGCCGACCTGTTCGAAACGACCGCCGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGGCCGATGCGCAACGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0N7JQB8

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References