Detailed information of TA system TA057793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1008970..1009664
• Replicon: chromosome
• Accession: NZ_CP013031
• Organism: Escherichia coli strain H1827/12

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: AB847_RS04885
• Protein ID: WP_001263493.1
• Coordinates: 1009266..1009664 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AB847_RS04880
• Protein ID: WP_000554757.1
• Coordinates: 1008970..1009263 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB847_RS04860	1004610..1005107	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
AB847_RS04865	1005323..1007035	-	1713	Protein_903	flagellar biosynthesis protein FlhA	-
AB847_RS04870	1007007..1007792	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
AB847_RS04875	1007863..1008918	+	1056	WP_001226164.1	DNA polymerase IV	-
AB847_RS04880	1008970..1009263	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AB847_RS04885	1009266..1009664	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AB847_RS04890	1009674..1010126	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
AB847_RS04895	1010372..1010575	+	204	Protein_909	RNA ligase RtcB family protein	-
AB847_RS04900	1010574..1011095	+	522	Protein_910	peptide chain release factor H	-
AB847_RS04905	1011152..1012609	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
AB847_RS04910	1012870..1013328	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AB847_RS04915	1013420..1014664	+	1245	WP_000189541.1	esterase FrsA	-
-	1013924..1014004	+	81	NuclAT_13	-	-
-	1013924..1014004	+	81	NuclAT_13	-	-
-	1013924..1014004	+	81	NuclAT_13	-	-
-	1013924..1014004	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T57793 WP_001263493.1 NZ_CP013031:1009266-1009664 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T57793 NZ_CP013031:1009266-1009664 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT57793 WP_000554757.1 NZ_CP013031:1008970-1009263 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT57793 NZ_CP013031:1008970-1009263 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6