Detailed information of TA system TA057785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4221842..4222641
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: AKK22_RS22580
• Protein ID: WP_032208138.1
• Coordinates: 4222177..4222641 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: AKK22_RS22575
• Protein ID: WP_032208136.1
• Coordinates: 4221842..4222177 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS22560	4217627..4218397	-	771	WP_032208131.1	2-dehydro-3-deoxyglucarate aldolase	-
AKK22_RS22565	4218413..4219747	-	1335	WP_032208133.1	galactarate/glucarate/glycerate transporter GarP	-
AKK22_RS22570	4220122..4221693	+	1572	WP_032208135.1	galactarate dehydratase	-
AKK22_RS22575	4221842..4222177	+	336	WP_032208136.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AKK22_RS22580	4222177..4222641	+	465	WP_032208138.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AKK22_RS22585	4222696..4223505	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AKK22_RS22590	4223754..4225034	+	1281	WP_000681922.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AKK22_RS22595	4225057..4225530	+	474	WP_032208140.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AKK22_RS22600	4225541..4226320	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
AKK22_RS22605	4226310..4227188	+	879	WP_001341890.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
AKK22_RS22610	4227206..4227640	+	435	WP_032208141.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4211198..4222641	11443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17837.19 Da
• Isoelectric Point: 9.4947

>T57785 WP_032208138.1 NZ_CP013029:4222177-4222641 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGASRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T57785 NZ_CP013029:4222177-4222641 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTAGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12405.01 Da
• Isoelectric Point: 4.8616

>AT57785 WP_032208136.1 NZ_CP013029:4221842-4222177 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKLGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT57785 NZ_CP013029:4221842-4222177 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCTAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA