Detailed information of TA system TA057781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3717180..3717907
• Replicon: chromosome
• Accession: NZ_CP013029
• Organism: Escherichia coli strain 2012C-4227

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AKK22_RS20060
• Protein ID: WP_000547563.1
• Coordinates: 3717596..3717907 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AKK22_RS20055
• Protein ID: WP_000126296.1
• Coordinates: 3717180..3717599 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKK22_RS20035	3712229..3712756	-	528	WP_032207572.1	electron transport protein HydN	-
AKK22_RS20040	3712905..3713915	-	1011	WP_001374632.1	DNA-binding transcriptional regulator AscG	-
AKK22_RS20045	3714175..3715632	+	1458	WP_032207573.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AKK22_RS20050	3715641..3717065	+	1425	WP_032207736.1	6-phospho-beta-glucosidase AscB	-
AKK22_RS20055	3717180..3717599	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
AKK22_RS20060	3717596..3717907	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AKK22_RS20065	3718069..3718824	-	756	WP_032207575.1	hypothetical protein	-
AKK22_RS20070	3718867..3719337	-	471	WP_032207577.1	hydrogenase maturation peptidase HycI	-
AKK22_RS20075	3719330..3719740	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AKK22_RS20080	3719737..3720504	-	768	WP_032207579.1	formate hydrogenlyase subunit HycG	-
AKK22_RS20085	3720504..3721046	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
AKK22_RS20090	3721056..3722765	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T57781 WP_000547563.1 NZ_CP013029:c3717907-3717596 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T57781 NZ_CP013029:c3717907-3717596 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT57781 WP_000126296.1 NZ_CP013029:c3717599-3717180 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT57781 NZ_CP013029:c3717599-3717180 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22