Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2989186..2989688 Replicon chromosome
Accession NZ_CP013029
Organism Escherichia coli strain 2012C-4227

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AKK22_RS16555 Protein ID WP_000767829.1
Coordinates 2989186..2989440 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AKK22_RS16560 Protein ID WP_001259255.1
Coordinates 2989437..2989688 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKK22_RS16530 2984201..2984428 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AKK22_RS16535 2984442..2985500 - 1059 WP_032206762.1 transport protein YeeE -
AKK22_RS16540 2985679..2987037 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AKK22_RS32155 2987027..2987089 - 63 WP_010723108.1 membrane protein YoeI -
AKK22_RS16545 2987304..2988233 - 930 WP_032206761.1 LysR family transcriptional regulator -
AKK22_RS16550 2988279..2989103 - 825 WP_000754749.1 SDR family oxidoreductase -
AKK22_RS16555 2989186..2989440 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AKK22_RS16560 2989437..2989688 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AKK22_RS29715 2989971..2990021 + 51 WP_001364200.1 his operon leader peptide -
AKK22_RS16565 2990167..2991066 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AKK22_RS16570 2991072..2992376 + 1305 WP_032206759.1 histidinol dehydrogenase -
AKK22_RS16575 2992373..2993443 + 1071 WP_000108943.1 histidinol-phosphate transaminase -
AKK22_RS16580 2993443..2994510 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T57779 WP_000767829.1 NZ_CP013029:c2989440-2989186 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T57779 NZ_CP013029:c2989440-2989186 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT57779 WP_001259255.1 NZ_CP013029:c2989688-2989437 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT57779 NZ_CP013029:c2989688-2989437 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References