Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 738054..738595 Replicon chromosome
Accession NZ_CP013029
Organism Escherichia coli strain 2012C-4227

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag AKK22_RS04305 Protein ID WP_000615979.1
Coordinates 738054..738332 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AKK22_RS04310 Protein ID WP_000729704.1
Coordinates 738335..738595 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKK22_RS04290 735636..736214 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AKK22_RS04295 736420..737187 + 768 WP_001365365.1 class II glutamine amidotransferase -
AKK22_RS04300 737158..737898 - 741 WP_001225679.1 murein L,D-transpeptidase -
AKK22_RS04305 738054..738332 - 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AKK22_RS04310 738335..738595 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AKK22_RS04315 738781..739554 + 774 WP_000543899.1 C40 family peptidase -
AKK22_RS04320 739611..739967 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AKK22_RS04325 739960..740238 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AKK22_RS04330 740343..742055 - 1713 Protein_724 flagellar biosynthesis protein FlhA -
AKK22_RS04335 742027..742812 + 786 WP_062875323.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 714334..746365 32031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T57764 WP_000615979.1 NZ_CP013029:c738332-738054 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T57764 NZ_CP013029:c738332-738054 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT57764 WP_000729704.1 NZ_CP013029:c738595-738335 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT57764 NZ_CP013029:c738595-738335 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References