Detailed information of TA system TA057755

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76374..76799
• Replicon: plasmid unnamed3
• Accession: NZ_CP013027
• Organism: Escherichia coli strain 2009C-3133

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: MJ49_RS30305
• Protein ID: WP_001332444.1
• Coordinates: 76638..76799 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	76374..76585 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ49_RS27305	71587..72006	-	420	WP_015056142.1	hypothetical protein	-
MJ49_RS27315	72307..72939	+	633	WP_000290818.1	single-stranded DNA-binding protein	-
MJ49_RS27320	72902..73135	+	234	WP_000005988.1	DUF905 family protein	-
MJ49_RS27325	73199..75157	+	1959	WP_000117202.1	ParB/RepB/Spo0J family partition protein	-
MJ49_RS27330	75212..75646	+	435	WP_000845922.1	conjugation system SOS inhibitor PsiB	-
MJ49_RS27335	75643..76362	+	720	WP_000994098.1	plasmid SOS inhibition protein A	-
-	76374..76585	+	212	NuclAT_0	-	Antitoxin
-	76374..76585	+	212	NuclAT_0	-	Antitoxin
-	76374..76585	+	212	NuclAT_0	-	Antitoxin
-	76374..76585	+	212	NuclAT_0	-	Antitoxin
MJ49_RS30305	76638..76799	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
MJ49_RS32825	77100..77397	-	298	Protein_79	hypothetical protein	-
MJ49_RS27350	77698..77985	+	288	WP_000107520.1	hypothetical protein	-
MJ49_RS27355	78104..78925	+	822	WP_001234468.1	DUF945 domain-containing protein	-
MJ49_RS27360	79221..79823	-	603	WP_077870411.1	transglycosylase SLT domain-containing protein	-
MJ49_RS27365	80144..80527	+	384	WP_001151528.1	relaxosome protein TraM	-
MJ49_RS27370	80714..81403	+	690	WP_000283383.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul2 / tet(B) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B	espP / iucA / iucB / iucC / iucD / iutA / espP	1..174564	174564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T57755 WP_001332444.1 NZ_CP013027:76638-76799 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T57755 NZ_CP013027:76638-76799 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT57755 NZ_CP013027:76374-76585 [Escherichia coli]
TCACACGGATTACCCGTGAACTGGTTGAACGACCAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin