Detailed information of TA system TA057751

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42064..42490
• Replicon: plasmid unnamed2
• Accession: NZ_CP013026
• Organism: Escherichia coli strain 2009C-3133

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MJ49_RS26760
• Protein ID: WP_001312861.1
• Coordinates: 42064..42222 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42266..42490 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ49_RS26730	37115..37342	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
MJ49_RS26735	37436..38122	-	687	WP_000332484.1	PAS domain-containing protein	-
MJ49_RS26740	38313..38696	-	384	WP_000124981.1	relaxosome protein TraM	-
MJ49_RS26745	38973..39620	+	648	WP_000614934.1	transglycosylase SLT domain-containing protein	-
MJ49_RS26750	39917..40738	-	822	WP_021573168.1	DUF945 domain-containing protein	-
MJ49_RS26755	40855..41142	-	288	WP_000107538.1	hypothetical protein	-
MJ49_RS32640	41498..41710	+	213	WP_162491396.1	hypothetical protein	-
MJ49_RS26760	42064..42222	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	42266..42490	-	225	NuclAT_0	-	Antitoxin
-	42266..42490	-	225	NuclAT_0	-	Antitoxin
-	42266..42490	-	225	NuclAT_0	-	Antitoxin
-	42266..42490	-	225	NuclAT_0	-	Antitoxin
MJ49_RS26770	42502..43221	-	720	WP_058820195.1	plasmid SOS inhibition protein A	-
MJ49_RS26775	43218..43652	-	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
MJ49_RS26780	43707..45665	-	1959	WP_060565196.1	ParB/RepB/Spo0J family partition protein	-
MJ49_RS26785	45730..45963	-	234	WP_000006030.1	DUF905 family protein	-
MJ49_RS26790	46019..46558	-	540	WP_060565198.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..63800	63800

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T57751 WP_001312861.1 NZ_CP013026:c42222-42064 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T57751 NZ_CP013026:c42222-42064 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT57751 NZ_CP013026:c42490-42266 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin