Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2018685..2019287 Replicon chromosome
Accession NZ_CP013025
Organism Escherichia coli strain 2009C-3133

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag MJ49_RS10895 Protein ID WP_000897305.1
Coordinates 2018976..2019287 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MJ49_RS10890 Protein ID WP_000356397.1
Coordinates 2018685..2018975 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ49_RS10870 2015187..2016089 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
MJ49_RS10875 2016086..2016721 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
MJ49_RS10880 2016718..2017647 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
MJ49_RS10885 2017862..2018080 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
MJ49_RS10890 2018685..2018975 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
MJ49_RS10895 2018976..2019287 - 312 WP_000897305.1 hypothetical protein Toxin
MJ49_RS10900 2019516..2020424 + 909 WP_001318161.1 alpha/beta hydrolase -
MJ49_RS10905 2020488..2021429 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
MJ49_RS10910 2021474..2021911 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
MJ49_RS10915 2021908..2022780 - 873 WP_000920762.1 virulence factor BrkB family protein -
MJ49_RS10920 2022774..2023373 - 600 WP_001315111.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T57726 WP_000897305.1 NZ_CP013025:c2019287-2018976 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T57726 NZ_CP013025:c2019287-2018976 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT57726 WP_000356397.1 NZ_CP013025:c2018975-2018685 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT57726 NZ_CP013025:c2018975-2018685 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References