Detailed information of TA system TA057712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 885608..886223
• Replicon: chromosome
• Accession: NZ_CP013013
• Organism: Vibrio cholerae strain Env-390

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: AP033_RS04135
• Protein ID: WP_001162670.1
• Coordinates: 885936..886223 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AP033_RS04130
• Protein ID: WP_001232701.1
• Coordinates: 885608..885925 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AP033_RS04105	880757..881632	-	876	WP_000259044.1	hypothetical protein	-
AP033_RS04115	882079..882555	-	477	WP_148661834.1	hypothetical protein	-
AP033_RS04120	882612..883442	-	831	WP_000056616.1	abortive infection family protein	-
AP033_RS04125	883647..884387	-	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
AP033_RS19840	884611..884649	-	39	WP_106019119.1	hypothetical protein	-
AP033_RS19205	884665..884790	-	126	WP_001944767.1	DUF645 family protein	-
AP033_RS19215	885136..885306	-	171	WP_001882300.1	DUF645 family protein	-
AP033_RS20055	885501..885611	-	111	WP_134814058.1	DUF3265 domain-containing protein	-
AP033_RS04130	885608..885925	-	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
AP033_RS04135	885936..886223	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AP033_RS19230	886490..886670	-	181	Protein_817	DUF645 family protein	-
AP033_RS19235	887095..887328	-	234	Protein_818	DUF3709 domain-containing protein	-
AP033_RS04140	887731..887949	-	219	WP_001917086.1	DUF3709 domain-containing protein	-
AP033_RS04145	888259..888912	-	654	WP_000226874.1	hypothetical protein	-
AP033_RS04150	888969..889478	-	510	WP_000405986.1	GNAT family N-acetyltransferase	-
AP033_RS04155	889720..889962	+	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
AP033_RS04160	889981..890298	+	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AP033_RS19245	890313..890435	-	123	WP_080033517.1	DUF3265 domain-containing protein	-
AP033_RS04165	890457..890675	-	219	WP_001209280.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	829699..942463	112764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T57712 WP_001162670.1 NZ_CP013013:c886223-885936 [Vibrio cholerae]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T57712 NZ_CP013013:c886223-885936 [Vibrio cholerae]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT57712 WP_001232701.1 NZ_CP013013:c885925-885608 [Vibrio cholerae]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT57712 NZ_CP013013:c885925-885608 [Vibrio cholerae]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36