Detailed information of TA system TA057678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3060163..3060823
• Replicon: chromosome
• Accession: NZ_CP012999
• Organism: Enterobacter sp. E20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: NI40_RS14320
• Protein ID: WP_039263808.1
• Coordinates: 3060470..3060823 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: NI40_RS14315
• Protein ID: WP_029741460.1
• Coordinates: 3060163..3060465 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NI40_RS14295	3056017..3058206	+	2190	WP_045142987.1	TonB-dependent siderophore receptor	-
NI40_RS14305	3058636..3059025	-	390	WP_045142988.1	RidA family protein	-
NI40_RS14310	3059167..3060087	+	921	WP_039263806.1	LysR family transcriptional regulator	-
NI40_RS14315	3060163..3060465	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
NI40_RS14320	3060470..3060823	-	354	WP_039263808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NI40_RS14325	3061001..3061894	-	894	WP_039263809.1	LysR family transcriptional regulator	-
NI40_RS14330	3062065..3062673	+	609	WP_080338147.1	glutathione S-transferase family protein	-
NI40_RS14335	3062717..3063667	-	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
NI40_RS14340	3063764..3064681	-	918	WP_033145933.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13586.44 Da
• Isoelectric Point: 9.7160

>T57678 WP_039263808.1 NZ_CP012999:c3060823-3060470 [Enterobacter sp. E20]
VWAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNRLKERE

Nucleotide

• Length: 354 bp

>T57678 NZ_CP012999:c3060823-3060470 [Enterobacter sp. E20]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGAACGCGCCAGCGTACTTGC
AGCACTGCTGGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGAATAGATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT57678 WP_029741460.1 NZ_CP012999:c3060465-3060163 [Enterobacter sp. E20]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT57678 NZ_CP012999:c3060465-3060163 [Enterobacter sp. E20]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCTGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79