Detailed information of TA system TA057648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 124672..125346
• Replicon: plasmid pKpN06-CTX
• Accession: NZ_CP012993
• Organism: Klebsiella pneumoniae strain KpN06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: AQD73_RS27300
• Protein ID: WP_032720638.1
• Coordinates: 124672..124995 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AQD73_RS27305
• Protein ID: WP_032720637.1
• Coordinates: 125044..125346 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD73_RS27270	119729..120127	+	399	Protein_142	recombinase family protein	-
AQD73_RS27275	120174..120824	+	651	WP_015065500.1	SOS response-associated peptidase	-
AQD73_RS27280	121121..122116	+	996	WP_019725651.1	hypothetical protein	-
AQD73_RS27285	122189..122512	+	324	WP_019725650.1	hypothetical protein	-
AQD73_RS27290	123013..123615	+	603	Protein_146	transposase	-
AQD73_RS30225	123942..124494	+	553	Protein_147	DUF4113 domain-containing protein	-
AQD73_RS27300	124672..124995	+	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQD73_RS27305	125044..125346	+	303	WP_032720637.1	helix-turn-helix domain-containing protein	Antitoxin
AQD73_RS31545	125389..125556	-	168	WP_153591440.1	hypothetical protein	-
AQD73_RS27310	125744..125923	-	180	WP_032720701.1	hypothetical protein	-
AQD73_RS27315	125947..126330	-	384	WP_032720636.1	hypothetical protein	-
AQD73_RS27320	126435..127007	-	573	WP_032720635.1	GNAT family N-acetyltransferase	-
AQD73_RS27325	127009..127797	-	789	WP_032720700.1	TSUP family transporter	-
AQD73_RS30860	127833..128753	-	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
AQD73_RS27335	129056..129550	-	495	WP_044266755.1	hypothetical protein	-
AQD73_RS27340	129581..130153	-	573	WP_044266753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..190072	190072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T57648 WP_032720638.1 NZ_CP012993:124672-124995 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T57648 NZ_CP012993:124672-124995 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT57648 WP_032720637.1 NZ_CP012993:125044-125346 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT57648 NZ_CP012993:125044-125346 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5