Detailed information of TA system TA057624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 124672..125346
• Replicon: plasmid pKpN01-CTX
• Accession: NZ_CP012988
• Organism: Klebsiella pneumoniae strain KpN01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LMS5
• Locus tag: AQD68_RS27290
• Protein ID: WP_032720638.1
• Coordinates: 124672..124995 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AQD68_RS27295
• Protein ID: WP_032720637.1
• Coordinates: 125044..125346 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AQD68_RS27260	119729..120127	+	399	Protein_142	recombinase family protein	-
AQD68_RS27265	120174..120824	+	651	WP_015065500.1	SOS response-associated peptidase	-
AQD68_RS27270	121121..122116	+	996	WP_019725651.1	hypothetical protein	-
AQD68_RS27275	122189..122512	+	324	WP_019725650.1	hypothetical protein	-
AQD68_RS27280	123013..123615	+	603	Protein_146	transposase	-
AQD68_RS30345	123942..124494	+	553	Protein_147	DUF4113 domain-containing protein	-
AQD68_RS27290	124672..124995	+	324	WP_032720638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AQD68_RS27295	125044..125346	+	303	WP_032720637.1	helix-turn-helix domain-containing protein	Antitoxin
AQD68_RS32205	125389..125556	-	168	WP_153591440.1	hypothetical protein	-
AQD68_RS27300	125744..125923	-	180	WP_032720701.1	hypothetical protein	-
AQD68_RS27305	125947..126330	-	384	WP_032720636.1	hypothetical protein	-
AQD68_RS27310	126435..127007	-	573	WP_032720635.1	GNAT family N-acetyltransferase	-
AQD68_RS27315	127009..127797	-	789	WP_032720700.1	TSUP family transporter	-
AQD68_RS30985	127833..128753	-	921	WP_050548604.1	TauD/TfdA family dioxygenase	-
AQD68_RS27325	129056..129550	-	495	WP_044266755.1	hypothetical protein	-
AQD68_RS27330	129581..130153	-	573	WP_044266753.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..190072	190072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.29 Da
• Isoelectric Point: 9.6240

>T57624 WP_032720638.1 NZ_CP012988:124672-124995 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T57624 NZ_CP012988:124672-124995 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11211.12 Da
• Isoelectric Point: 7.3611

>AT57624 WP_032720637.1 NZ_CP012988:125044-125346 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Nucleotide

• Length: 303 bp

>AT57624 NZ_CP012988:125044-125346 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LMS5