Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1338218..1338815 Replicon chromosome
Accession NZ_CP012987
Organism Klebsiella pneumoniae strain KpN01

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AQD68_RS06730 Protein ID WP_004142563.1
Coordinates 1338498..1338815 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AQD68_RS06725 Protein ID WP_004142561.1
Coordinates 1338218..1338505 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AQD68_RS06695 1334298..1334546 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AQD68_RS06700 1334564..1334905 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AQD68_RS06705 1334936..1336051 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
AQD68_RS06710 1336231..1336812 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
AQD68_RS06715 1336812..1337180 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AQD68_RS06720 1337300..1337953 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
AQD68_RS06725 1338218..1338505 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AQD68_RS06730 1338498..1338815 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AQD68_RS06735 1339000..1340043 - 1044 WP_048299257.1 DUF2157 domain-containing protein -
AQD68_RS06740 1340708..1341574 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
AQD68_RS06745 1341683..1343110 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T57606 WP_004142563.1 NZ_CP012987:c1338815-1338498 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T57606 NZ_CP012987:c1338815-1338498 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACTCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT57606 WP_004142561.1 NZ_CP012987:c1338505-1338218 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT57606 NZ_CP012987:c1338505-1338218 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References