Detailed information of TA system TA057506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2349935..2350501
• Replicon: chromosome
• Accession: NZ_CP012959
• Organism: Aggregatibacter actinomycetemcomitans strain 624

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: ACT75_RS11460
• Protein ID: WP_005543444.1
• Coordinates: 2350223..2350501 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ACT75_RS11455
• Protein ID: WP_005543446.1
• Coordinates: 2349935..2350207 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACT75_RS11430	2346470..2346736	-	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-
ACT75_RS12265	2347097..2347288	+	192	WP_005553960.1	hypothetical protein	-
ACT75_RS11435	2347263..2347640	+	378	WP_005543455.1	hypothetical protein	-
ACT75_RS11440	2347764..2348039	-	276	WP_005543453.1	hydrogenase maturation factor HybG	-
ACT75_RS12625	2348069..2348357	-	289	Protein_2261	[NiFe]-hydrogenase assembly chaperone HybE	-
ACT75_RS11450	2348496..2349926	-	1431	WP_005543449.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
ACT75_RS11455	2349935..2350207	-	273	WP_005543446.1	HigA family addiction module antidote protein	Antitoxin
ACT75_RS11460	2350223..2350501	-	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACT75_RS11465	2350596..2351597	-	1002	WP_005543441.1	anthranilate phosphoribosyltransferase	-
ACT75_RS11470	2351609..2352001	-	393	WP_005543439.1	tautomerase family protein	-
ACT75_RS11475	2352075..2352665	-	591	WP_005543437.1	aminodeoxychorismate/anthranilate synthase component II	-
ACT75_RS11480	2352676..2354223	-	1548	WP_005543435.1	anthranilate synthase component 1	-
ACT75_RS11485	2354389..2354943	-	555	WP_005543432.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T57506 WP_005543444.1 NZ_CP012959:c2350501-2350223 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T57506 NZ_CP012959:c2350501-2350223 [Aggregatibacter actinomycetemcomitans]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT57506 WP_005543446.1 NZ_CP012959:c2350207-2349935 [Aggregatibacter actinomycetemcomitans]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT57506 NZ_CP012959:c2350207-2349935 [Aggregatibacter actinomycetemcomitans]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Structures

Toxin

• AlphaFold DB: X2JU35