Detailed information of TA system TA057502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 912568..913156
• Replicon: chromosome
• Accession: NZ_CP012959
• Organism: Aggregatibacter actinomycetemcomitans strain 624

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5D0ELR5
• Locus tag: ACT75_RS04385
• Protein ID: WP_005541261.1
• Coordinates: 912881..913156 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACT75_RS04380
• Protein ID: WP_005541263.1
• Coordinates: 912568..912864 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACT75_RS04355	907966..909009	-	1044	WP_005541273.1	glycosyltransferase family 9 protein	-
ACT75_RS04360	909098..909823	+	726	WP_005541271.1	3-deoxy-D-manno-octulosonic acid kinase	-
ACT75_RS04365	909872..910366	-	495	WP_005541270.1	pantetheine-phosphate adenylyltransferase	-
ACT75_RS04370	910367..911650	-	1284	WP_005541268.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
ACT75_RS04375	911749..912510	+	762	WP_005541265.1	glycosyltransferase family 2 protein	-
ACT75_RS04380	912568..912864	-	297	WP_005541263.1	HigA family addiction module antidote protein	Antitoxin
ACT75_RS04385	912881..913156	-	276	WP_005541261.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACT75_RS04390	913273..914640	-	1368	WP_005541259.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
ACT75_RS04395	914826..915902	+	1077	WP_005551449.1	tRNA/rRNA methyltransferase	-
ACT75_RS04400	915969..916229	-	261	WP_032998892.1	YfhL family 4Fe-4S dicluster ferredoxin	-
ACT75_RS04405	916297..916992	+	696	WP_005541254.1	ribonuclease T2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-85)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10595.09 Da
• Isoelectric Point: 8.0554

>T57502 WP_005541261.1 NZ_CP012959:c913156-912881 [Aggregatibacter actinomycetemcomitans]
MIKSFKHKGLQAYFETGVTKGIQSAHKKKINGILDLIDNATSETEFKMFYQCHPLKGDREGIWSMKVSGNWRITFEFING
DAYILNYEDYH

Nucleotide

• Length: 276 bp

>T57502 NZ_CP012959:c913156-912881 [Aggregatibacter actinomycetemcomitans]
ATGATTAAGAGTTTTAAACATAAAGGATTACAAGCCTATTTTGAAACCGGTGTAACCAAAGGGATTCAATCTGCACACAA
AAAGAAAATCAACGGTATTCTTGATTTAATTGATAATGCAACTTCTGAAACGGAATTTAAGATGTTTTACCAATGCCATC
CGTTAAAAGGTGACAGAGAAGGAATTTGGTCAATGAAAGTATCGGGTAATTGGCGTATTACTTTTGAGTTTATTAACGGT
GATGCGTATATATTAAACTATGAAGATTATCATTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11099.77 Da
• Isoelectric Point: 7.4038

>AT57502 WP_005541263.1 NZ_CP012959:c912864-912568 [Aggregatibacter actinomycetemcomitans]
MRKPAHPGEILLDGFIEPHDIKIKDLAEHLGFSRETLSRVIHGKTPMTANLALALEDAGISTARLWLSLQSNYDVWALKQ
TREASTIRPFVFSEQRFV

Nucleotide

• Length: 297 bp

>AT57502 NZ_CP012959:c912864-912568 [Aggregatibacter actinomycetemcomitans]
ATGCGTAAACCTGCACATCCGGGCGAAATTTTATTAGACGGTTTTATTGAACCACATGACATTAAGATTAAAGATTTAGC
CGAGCATTTAGGTTTTTCCCGTGAAACGCTTTCGCGGGTCATTCACGGCAAAACGCCAATGACTGCAAATTTAGCGTTGG
CATTGGAGGATGCCGGCATCAGTACCGCCCGTTTATGGCTTTCATTACAAAGCAATTATGATGTTTGGGCATTGAAACAA
ACACGGGAAGCATCCACAATCCGGCCTTTTGTATTCTCGGAACAACGTTTTGTATAA

Structures

Toxin

• AlphaFold DB: A0A5D0ELR5