Detailed information of TA system TA057494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 778020..778586
• Replicon: chromosome
• Accession: NZ_CP012958
• Organism: Aggregatibacter actinomycetemcomitans strain VT1169

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JU35
• Locus tag: ACT74_RS03660
• Protein ID: WP_005543444.1
• Coordinates: 778020..778298 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: ACT74_RS03665
• Protein ID: WP_005543446.1
• Coordinates: 778314..778586 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACT74_RS03635	773576..774130	+	555	WP_005543432.1	HAD family hydrolase	-
ACT74_RS03640	774298..775845	+	1548	WP_005572118.1	anthranilate synthase component 1	-
ACT74_RS03645	775856..776446	+	591	WP_005572120.1	aminodeoxychorismate/anthranilate synthase component II	-
ACT74_RS03650	776520..776912	+	393	WP_005543439.1	tautomerase family protein	-
ACT74_RS03655	776924..777925	+	1002	WP_061869877.1	anthranilate phosphoribosyltransferase	-
ACT74_RS03660	778020..778298	+	279	WP_005543444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACT74_RS03665	778314..778586	+	273	WP_005543446.1	HigA family addiction module antidote protein	Antitoxin
ACT74_RS03670	778595..780025	+	1431	WP_061869878.1	bifunctional indole-3-glycerol-phosphate synthase TrpC/phosphoribosylanthranilate isomerase TrpF	-
ACT74_RS03675	780480..780755	+	276	WP_005561117.1	hydrogenase maturation factor HybG	-
ACT74_RS03680	780876..781253	-	378	WP_005543455.1	hypothetical protein	-
ACT74_RS10545	781228..781419	-	192	WP_005553960.1	hypothetical protein	-
ACT74_RS03685	781779..782045	+	267	WP_005543457.1	oxaloacetate decarboxylase subunit gamma	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-79)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 9.5983

>T57494 WP_005543444.1 NZ_CP012958:778020-778298 [Aggregatibacter actinomycetemcomitans]
MILSFKHKGLEQFFKTGSTAGIQAKHAKKLNLQLATLNNAETPLAMSVPSWNLHKLKGDLQEHWAVTVNGNWRLTFKFEN
GHAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T57494 NZ_CP012958:778020-778298 [Aggregatibacter actinomycetemcomitans]
ATGATTTTATCTTTCAAACATAAGGGACTTGAACAATTTTTTAAAACCGGTTCGACCGCAGGAATTCAAGCCAAACACGC
CAAGAAATTAAATCTGCAATTGGCCACATTAAATAATGCGGAAACGCCTCTTGCTATGAGTGTTCCCAGCTGGAATTTGC
ACAAACTTAAAGGTGATTTGCAAGAGCATTGGGCAGTTACCGTAAACGGAAATTGGCGCTTAACATTTAAATTTGAAAAT
GGTCATGCTGAAGTTGTTGATTATCAGGATTATCATTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10138.74 Da
• Isoelectric Point: 7.3169

>AT57494 WP_005543446.1 NZ_CP012958:778314-778586 [Aggregatibacter actinomycetemcomitans]
MHNPPHPGELLKEYIDGVSVTKVAQKLGVSRVTLSRILNGKASITPEMAVRLSELLNTTTPKLWLGMQADYDLWQIEQQH
AVFNIQPLFN

Nucleotide

• Length: 273 bp

>AT57494 NZ_CP012958:778314-778586 [Aggregatibacter actinomycetemcomitans]
ATGCACAATCCTCCACACCCGGGAGAACTGTTAAAAGAATATATTGATGGCGTGAGTGTCACGAAGGTTGCCCAAAAATT
AGGTGTTTCGCGTGTTACGCTTTCCCGCATTCTTAATGGGAAAGCAAGTATAACGCCTGAAATGGCTGTGCGATTAAGCG
AATTATTGAATACCACAACACCGAAATTATGGCTGGGTATGCAAGCAGACTACGATTTATGGCAAATTGAACAACAACAC
GCCGTATTCAACATCCAACCATTATTTAATTAA

Structures

Toxin

• AlphaFold DB: X2JU35