Detailed information of TA system TA057491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 529128..529774
• Replicon: chromosome
• Accession: NZ_CP012958
• Organism: Aggregatibacter actinomycetemcomitans strain VT1169

Toxin (Protein)

• Gene name: higB
• Uniprot ID: X2JQ23
• Locus tag: ACT74_RS02545
• Protein ID: WP_005555126.1
• Coordinates: 529128..529478 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACT74_RS02550
• Protein ID: WP_061869849.1
• Coordinates: 529475..529774 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACT74_RS02525	524306..524551	-	246	WP_005540815.1	YgjV family protein	-
ACT74_RS02530	524741..526078	-	1338	WP_005567415.1	argininosuccinate synthase	-
ACT74_RS02535	526173..527000	-	828	WP_005540818.1	hypothetical protein	-
ACT74_RS02540	527357..528940	+	1584	WP_005567417.1	peptide chain release factor 3	-
ACT74_RS02545	529128..529478	+	351	WP_005555126.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ACT74_RS02550	529475..529774	+	300	WP_061869849.1	helix-turn-helix transcriptional regulator	Antitoxin
ACT74_RS02555	529810..532743	-	2934	WP_014167733.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-
ACT74_RS02560	532945..533172	+	228	WP_005567423.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13518.64 Da
• Isoelectric Point: 9.9664

>T57491 WP_005555126.1 NZ_CP012958:529128-529478 [Aggregatibacter actinomycetemcomitans]
MWAVILTERFETWLNQQPIKTQECVLANLVRLEHFGPNLARPYVDSVAGSIHSNMKELRVQHSGRAIRLFFAFDPKRRAI
VLCGGDKGNDKRFYRTMIRIADQELNHYLATMEQTK

Nucleotide

• Length: 351 bp

>T57491 NZ_CP012958:529128-529478 [Aggregatibacter actinomycetemcomitans]
ATGTGGGCGGTGATTTTAACGGAACGATTTGAAACTTGGTTAAATCAACAGCCGATAAAAACGCAGGAATGTGTTTTGGC
AAACTTGGTACGGTTGGAACATTTTGGTCCGAATTTAGCCCGTCCTTATGTAGATAGCGTAGCAGGGTCAATTCATAGCA
ACATGAAAGAACTACGCGTTCAGCATTCGGGAAGAGCAATTCGACTCTTTTTTGCTTTTGATCCAAAGCGTAGAGCCATT
GTTTTATGTGGTGGAGATAAAGGCAATGACAAGCGGTTTTACCGTACGATGATTAGAATTGCAGATCAAGAATTGAATCA
TTATTTGGCAACCATGGAGCAAACAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11376.14 Da
• Isoelectric Point: 5.8598

>AT57491 WP_061869849.1 NZ_CP012958:529475-529774 [Aggregatibacter actinomycetemcomitans]
MKTLQQVIDERSVESRQRIQTMAEDLILETGLQLLREELNISQQQLATILGVTQPAINQLEQRGNEIKLATFKRYIEAMG
GKLRLSVEMPDGHQRVFQI

Nucleotide

• Length: 300 bp

>AT57491 NZ_CP012958:529475-529774 [Aggregatibacter actinomycetemcomitans]
ATGAAAACATTACAACAAGTTATTGATGAACGTTCGGTAGAAAGCCGCCAACGTATTCAAACCATGGCGGAAGATTTAAT
TCTTGAAACCGGTTTGCAACTGTTGCGAGAAGAATTAAATATATCGCAACAACAATTAGCTACGATTTTAGGTGTAACTC
AACCTGCAATTAATCAACTGGAACAGCGTGGCAATGAAATTAAGCTTGCAACCTTCAAACGTTACATCGAAGCAATGGGC
GGCAAATTACGCCTTAGTGTAGAAATGCCTGATGGACATCAGCGGGTTTTTCAAATTTAA

Structures

Toxin

• AlphaFold DB: X2JQ23