Detailed information of TA system TA057488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4625..5276
• Replicon: plasmid pD36-3 clone GC1
• Accession: NZ_CP012955
• Organism: Acinetobacter baumannii strain D36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4R0DYU1
• Locus tag: AN415_RS19535
• Protein ID: WP_000269907.1
• Coordinates: 4625..4981 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN415_RS19540
• Protein ID: WP_001140617.1
• Coordinates: 4974..5276 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN415_RS19515	140..1090	+	951	WP_001205343.1	replication initiation protein RepM	-
AN415_RS19520	1083..1658	+	576	WP_001096616.1	helix-turn-helix domain-containing protein	-
AN415_RS20900	1678..1821	+	144	WP_001125246.1	hypothetical protein	-
AN415_RS19525	2029..3483	-	1455	WP_005249159.1	SulP family inorganic anion transporter	-
AN415_RS19530	3732..3941	-	210	WP_005249157.1	hypothetical protein	-
AN415_RS19535	4625..4981	+	357	WP_000269907.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN415_RS19540	4974..5276	+	303	WP_001140617.1	helix-turn-helix transcriptional regulator	Antitoxin
AN415_RS19545	5399..5800	+	402	WP_000660211.1	hypothetical protein	-
AN415_RS19550	5817..6119	+	303	WP_001081086.1	hypothetical protein	-
AN415_RS19555	6184..6924	-	741	WP_031380848.1	DUF3800 domain-containing protein	-
AN415_RS19560	7235..7621	+	387	WP_001986792.1	helix-turn-helix domain-containing protein	-
AN415_RS20905	7658..7831	-	174	WP_001282484.1	hypothetical protein	-
AN415_RS19565	7831..8202	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9276	9276

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7172

>T57488 WP_000269907.1 NZ_CP012955:4625-4981 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYTEMLAIADQQYELHLSTLGEQSNG

Nucleotide

• Length: 357 bp

>T57488 NZ_CP012955:4625-4981 [Acinetobacter baumannii]
ATGTGGACAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGATGAATCAACACAAGAGAAGGTTTTAGC
TGCTTTGGTTGTTCTGCAACAACAGGGACCGAGCTTAGGCCGCCCTTTAGTAGATACCGTGTATGACTCTAAATTTACCA
ATATGAAAGAGCTTCGTGTTCAACATCGTGGTAAACCACTCCGAGCTTTTTTTGCATTTGATCCATTACGACAGGCCATT
GTGCTGTGTATTGGTGATAAAGGTGGTAAAAAGCGATTCTATACAGAGATGCTGGCGATTGCAGATCAACAATATGAACT
TCATCTCTCAACATTAGGAGAACAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11262.05 Da
• Isoelectric Point: 5.8961

>AT57488 WP_001140617.1 NZ_CP012955:4974-5276 [Acinetobacter baumannii]
MAKTLQELLANRSPESQARIQKIADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT57488 NZ_CP012955:4974-5276 [Acinetobacter baumannii]
ATGGCTAAAACTTTGCAAGAATTATTAGCTAACCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATAGCTGATGAATT
GCTATTAGAAAATCAGCTTTACCTTATTCGTGAAGAACTCGAAATCTCTCAAAAAGAACTGGCTGAAACTTTGGGAATAA
AACAGCCATCACTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATCTCAACCATGAAAAAGTATGTTGAAGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A4R0DYU1