Detailed information of TA system TA057485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2372977..2373491
• Replicon: chromosome
• Accession: NZ_CP012949
• Organism: Streptomyces ambofaciens strain DSM 40697

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A0K2AQU7
• Locus tag: SAM40697_RS10765
• Protein ID: WP_053129813.1
• Coordinates: 2373237..2373491 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: SAM40697_RS10760
• Protein ID: WP_063481883.1
• Coordinates: 2372977..2373240 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAM40697_RS10750	2368684..2369544	+	861	WP_063481882.1	phosphatase PAP2 family protein	-
SAM40697_RS10755	2369815..2372823	-	3009	WP_053129807.1	bifunctional [glutamine synthetase] adenylyltransferase/[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase	-
SAM40697_RS10760	2372977..2373240	+	264	WP_063481883.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SAM40697_RS10765	2373237..2373491	+	255	WP_053129813.1	Txe/YoeB family addiction module toxin	Toxin
SAM40697_RS10770	2373552..2373998	+	447	WP_063481884.1	VOC family protein	-
SAM40697_RS10775	2374025..2375779	-	1755	WP_063481885.1	NAD+ synthase	-
SAM40697_RS10780	2375834..2376460	-	627	WP_063481886.1	DUF305 domain-containing protein	-
SAM40697_RS10785	2376457..2377128	-	672	WP_053129825.1	DUF3105 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9999.29 Da
• Isoelectric Point: 9.3758

>T57485 WP_053129813.1 NZ_CP012949:2373237-2373491 [Streptomyces ambofaciens]
VRIAFTSHGWEDYVHWAESDRKVTRRINKLITDISRDPFKGIGKPEPLKGDLSGYWSRRIDDTHRLVYKPTDDELIIVQA
RYHY

Nucleotide

• Length: 255 bp

>T57485 NZ_CP012949:2373237-2373491 [Streptomyces ambofaciens]
GTGAGAATCGCTTTCACGTCGCACGGCTGGGAGGACTACGTCCACTGGGCCGAGAGCGACCGGAAGGTCACCAGACGGAT
CAACAAGCTGATCACCGACATCTCCCGCGACCCGTTCAAGGGCATCGGCAAGCCGGAGCCGCTGAAGGGCGACCTGTCGG
GCTACTGGTCACGCCGCATCGACGACACGCACCGTCTGGTCTACAAGCCCACCGACGACGAGCTGATCATCGTCCAGGCG
CGGTACCACTACTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9663.83 Da
• Isoelectric Point: 5.6227

>AT57485 WP_063481883.1 NZ_CP012949:2372977-2373240 [Streptomyces ambofaciens]
MSITASEARQNLFPLIEQVNEDHAPVHITSRKGNAVLMSEEDFTAWTETVHLLRSPKNARRLLDSIAEAEAGGARRRDLI
APDAERA

Nucleotide

• Length: 264 bp

>AT57485 NZ_CP012949:2372977-2373240 [Streptomyces ambofaciens]
ATGTCCATTACCGCCAGCGAAGCCCGTCAGAACCTGTTCCCGCTGATAGAGCAGGTCAACGAGGATCACGCCCCGGTGCA
CATCACCTCCCGCAAGGGAAACGCCGTGCTCATGTCGGAGGAGGACTTCACCGCCTGGACGGAGACGGTGCATCTGCTGC
GGTCGCCCAAGAACGCCCGCCGTCTGCTCGACTCGATCGCGGAGGCCGAGGCCGGCGGCGCGCGGCGGCGCGACCTGATC
GCCCCGGACGCGGAGCGGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K2AQU7