Detailed information of TA system TA057474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1886854..1887491
• Replicon: plasmid unnamed
• Accession: NZ_CP012944
• Organism: Ralstonia solanacearum strain IBSBF1503

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6G785
• Locus tag: RALBFv3_RS22810
• Protein ID: WP_014618424.1
• Coordinates: 1887162..1887491 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RALBFv3_RS22805
• Protein ID: WP_003262799.1
• Coordinates: 1886854..1887165 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RALBFv3_RS22775	1882041..1882799	-	759	WP_042548869.1	hypothetical protein	-
RALBFv3_RS24770	1882809..1883123	-	315	WP_080773821.1	tyrosine-type recombinase/integrase	-
RALBFv3_RS22780	1883138..1883878	+	741	WP_003262792.1	metallophosphatase family protein	-
RALBFv3_RS22785	1884071..1884427	-	357	WP_042549721.1	hypothetical protein	-
RALBFv3_RS22790	1884724..1885557	+	834	WP_042548870.1	DUF2242 domain-containing protein	-
RALBFv3_RS25385	1885560..1885766	-	207	WP_014618427.1	hypothetical protein	-
RALBFv3_RS22795	1886139..1886450	+	312	WP_039551557.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
RALBFv3_RS22800	1886395..1886703	+	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
RALBFv3_RS22805	1886854..1887165	-	312	WP_003262799.1	transcriptional regulator	Antitoxin
RALBFv3_RS22810	1887162..1887491	-	330	WP_014618424.1	hypothetical protein	Toxin
RALBFv3_RS22815	1887542..1889491	-	1950	WP_042548871.1	propionate catabolism operon regulatory protein PrpR	-
RALBFv3_RS22820	1889685..1890581	+	897	WP_014618422.1	methylisocitrate lyase	-
RALBFv3_RS22825	1890619..1891782	+	1164	WP_014618421.1	2-methylcitrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliM / flgG / cyaB / cheY	1..2069788	2069788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12413.89 Da
• Isoelectric Point: 5.2566

>T57474 WP_014618424.1 NZ_CP012944:c1887491-1887162 [Ralstonia solanacearum]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEAADLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T57474 NZ_CP012944:c1887491-1887162 [Ralstonia solanacearum]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCGGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGCCGATCTTTCCAGTGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT57474 WP_003262799.1 NZ_CP012944:c1887165-1886854 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT57474 NZ_CP012944:c1887165-1886854 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGGCCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCCCATGCCT
CACAGGCTGTCATGGCACGTCGGTTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: F6G785