Detailed information of TA system TA057469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 1883187..1883824
• Replicon: plasmid unnamed
• Accession: NZ_CP012940
• Organism: Ralstonia solanacearum strain UW163

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6G785
• Locus tag: UW163_RS23430
• Protein ID: WP_014618424.1
• Coordinates: 1883495..1883824 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: UW163_RS23425
• Protein ID: WP_003262799.1
• Coordinates: 1883187..1883498 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UW163_RS25425	1879142..1879456	-	315	WP_080773821.1	tyrosine-type recombinase/integrase	-
UW163_RS23400	1879471..1880211	+	741	WP_003262792.1	metallophosphatase family protein	-
UW163_RS23405	1880404..1880760	-	357	WP_042549721.1	hypothetical protein	-
UW163_RS23410	1881057..1881890	+	834	WP_042548870.1	DUF2242 domain-containing protein	-
UW163_RS26140	1881893..1882099	-	207	WP_014618427.1	hypothetical protein	-
UW163_RS23415	1882472..1882783	+	312	WP_039551557.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
UW163_RS23420	1882728..1883036	+	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
UW163_RS23425	1883187..1883498	-	312	WP_003262799.1	transcriptional regulator	Antitoxin
UW163_RS23430	1883495..1883824	-	330	WP_014618424.1	hypothetical protein	Toxin
UW163_RS23435	1883875..1885824	-	1950	WP_042548871.1	propionate catabolism operon regulatory protein PrpR	-
UW163_RS23440	1886018..1886914	+	897	WP_014618422.1	methylisocitrate lyase	-
UW163_RS23445	1886952..1888115	+	1164	WP_014618421.1	2-methylcitrate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA / cyaB	1..1932001	1932001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12413.89 Da
• Isoelectric Point: 5.2566

>T57469 WP_014618424.1 NZ_CP012940:c1883824-1883495 [Ralstonia solanacearum]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEAADLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T57469 NZ_CP012940:c1883824-1883495 [Ralstonia solanacearum]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCGGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGCCGATCTTTCCAGTGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT57469 WP_003262799.1 NZ_CP012940:c1883498-1883187 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT57469 NZ_CP012940:c1883498-1883187 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGGCCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCCCATGCCT
CACAGGCTGTCATGGCACGTCGGTTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: F6G785