Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 2201569..2202155 Replicon chromosome
Accession NZ_CP012883
Organism Klebsiella pneumoniae KP-1

Toxin (Protein)


Gene name doc Uniprot ID A0A331ME34
Locus tag KLP1_RS11050 Protein ID WP_025861226.1
Coordinates 2201569..2201937 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KLP1_RS11055 Protein ID WP_004174006.1
Coordinates 2201934..2202155 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KLP1_RS11030 2197072..2198142 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
KLP1_RS11035 2198144..2198989 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KLP1_RS11040 2198986..2199873 - 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KLP1_RS11045 2199980..2201296 - 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KLP1_RS11050 2201569..2201937 - 369 WP_025861226.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KLP1_RS11055 2201934..2202155 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KLP1_RS11060 2202320..2203033 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KLP1_RS11065 2203035..2203802 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KLP1_RS11070 2203799..2205076 - 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KLP1_RS11075 2205073..2205999 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2196335..2205076 8741


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13551.98 Da        Isoelectric Point: 8.6410

>T57418 WP_025861226.1 NZ_CP012883:c2201937-2201569 [Klebsiella pneumoniae KP-1]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T57418 NZ_CP012883:c2201937-2201569 [Klebsiella pneumoniae KP-1]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTATTAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT57418 WP_004174006.1 NZ_CP012883:c2202155-2201934 [Klebsiella pneumoniae KP-1]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT57418 NZ_CP012883:c2202155-2201934 [Klebsiella pneumoniae KP-1]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331ME34

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References