Detailed information of TA system TA057392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4596613..4597343
• Replicon: chromosome
• Accession: NZ_CP012871
• Organism: [Enterobacter] lignolyticus strain G5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: E3G7Q4
• Locus tag: AO703_RS21400
• Protein ID: WP_013364087.1
• Coordinates: 4597029..4597343 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AO703_RS21395
• Protein ID: WP_013364088.1
• Coordinates: 4596613..4597032 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO703_RS21375	4592246..4593166	-	921	WP_013364092.1	ribokinase	-
AO703_RS21380	4593493..4594278	+	786	WP_062742384.1	DeoR family transcriptional regulator	-
AO703_RS21385	4594280..4594567	-	288	WP_013364090.1	acetolactate synthase small subunit	-
AO703_RS21390	4594571..4596259	-	1689	WP_062742385.1	acetolactate synthase large subunit	-
AO703_RS23270	4596364..4596465	-	102	WP_125452054.1	ilvB operon leader peptide IvbL	-
AO703_RS21395	4596613..4597032	-	420	WP_013364088.1	helix-turn-helix domain-containing protein	Antitoxin
AO703_RS21400	4597029..4597343	-	315	WP_013364087.1	type II toxin-antitoxin system HigB family toxin	Toxin
AO703_RS21405	4597643..4598827	+	1185	WP_013364086.1	multidrug efflux MFS transporter EmrD	-
AO703_RS21410	4598802..4599755	-	954	WP_062742386.1	DNA-binding transcriptional regulator DsdC	-
AO703_RS21415	4599983..4601320	+	1338	WP_013364084.1	D-serine transporter DsdX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12267.39 Da
• Isoelectric Point: 10.1475

>T57392 WP_013364087.1 NZ_CP012871:c4597343-4597029 [[Enterobacter] lignolyticus]
MHLISMKAILDAVNTFPHHKEELLLLGRVIEKGNCPTPAALKKLYPTLDNFKYLDKHYIIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTDKHRAKGKK

Nucleotide

• Length: 315 bp

>T57392 NZ_CP012871:c4597343-4597029 [[Enterobacter] lignolyticus]
ATGCATTTAATCTCAATGAAGGCTATTTTGGATGCGGTAAATACGTTTCCGCACCACAAGGAAGAGCTACTCCTTCTGGG
GCGAGTGATCGAAAAGGGAAATTGCCCGACGCCTGCTGCACTGAAAAAGCTCTATCCAACGCTGGATAACTTCAAGTATC
TGGATAAACATTATATTATTGATATTGCAAATAATAATCTTAGGGTGGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGATAAACATCGCGCTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15499.08 Da
• Isoelectric Point: 4.9534

>AT57392 WP_013364088.1 NZ_CP012871:c4597032-4596613 [[Enterobacter] lignolyticus]
MMIVADAIKATQALVAAVPLLGEHPNDKDYEEALELVEYLLMNEPRSALLDIVCAKISRYEANQPEIVALRQEMESVPVG
IAVLRTLMDQHKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAIRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT57392 NZ_CP012871:c4597032-4596613 [[Enterobacter] lignolyticus]
ATGATGATCGTCGCTGATGCAATTAAAGCTACCCAGGCTCTTGTTGCGGCTGTTCCCTTACTGGGCGAACATCCGAACGA
CAAAGATTATGAGGAAGCCCTTGAGCTGGTTGAGTATTTGCTCATGAACGAGCCACGCAGCGCTTTGCTGGATATCGTAT
GTGCCAAAATCAGCCGCTATGAAGCGAATCAACCCGAAATAGTGGCCTTAAGGCAGGAGATGGAGTCTGTTCCTGTCGGT
ATTGCGGTTTTAAGAACCCTTATGGATCAGCACAAACTGACTATTTCTGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCCAGGGTTCTTAATGGTCAGCGTCAGCTTACCTTAAATCATATTAAAAAGCTGGCGATCCGTTTTGGCGTGTCGC
CTGCGCTTTTCATAGAATGA

Structures

Toxin

• AlphaFold DB: A0A806X9T4