Detailed information of TA system TA057375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4388991..4389685
• Replicon: chromosome
• Accession: NZ_CP012870
• Organism: Escherichia coli strain K-12 substr. MG1655_TMP32XR2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: QR63_RS21725
• Protein ID: WP_001263489.1
• Coordinates: 4388991..4389389 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: QR63_RS21730
• Protein ID: WP_000554758.1
• Coordinates: 4389392..4389685 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4384579..4384659	-	81	NuclAT_12	-	-
-	4384579..4384659	-	81	NuclAT_12	-	-
-	4384579..4384659	-	81	NuclAT_12	-	-
-	4384579..4384659	-	81	NuclAT_12	-	-
QR63_RS21700	4385255..4385713	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
QR63_RS21705	4385974..4387431	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
QR63_RS21710	4387488..4388009	-	522	Protein_4136	peptide chain release factor H	-
QR63_RS21715	4388005..4388211	-	207	Protein_4137	RtcB family protein	-
QR63_RS21720	4388529..4388981	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
QR63_RS21725	4388991..4389389	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
QR63_RS21730	4389392..4389685	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
QR63_RS21735	4389737..4390792	-	1056	WP_001226164.1	DNA polymerase IV	-
QR63_RS21740	4390863..4391648	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
QR63_RS21745	4391620..4393332	+	1713	Protein_4143	flagellar biosynthesis protein FlhA	-
QR63_RS21750	4393556..4394053	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4344654..4394855	50201

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T57375 WP_001263489.1 NZ_CP012870:c4389389-4388991 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T57375 NZ_CP012870:c4389389-4388991 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT57375 WP_000554758.1 NZ_CP012870:c4389685-4389392 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT57375 NZ_CP012870:c4389685-4389392 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3