57329

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2551941..2552443 Replicon chromosome
Accession NZ_CP012869
Organism Escherichia coli strain K-12 substr. MG1655_TMP32XR1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag QR62_RS12400 Protein ID WP_000767829.1
Coordinates 2552189..2552443 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag QR62_RS12395 Protein ID WP_001259255.1
Coordinates 2551941..2552192 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QR62_RS12375 2547119..2548186 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
QR62_RS12380 2548186..2549256 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
QR62_RS12385 2549253..2550557 - 1305 WP_000009594.1 histidinol dehydrogenase -
QR62_RS12390 2550563..2551462 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
QR62_RS23950 2551608..2551658 - 51 WP_001364200.1 his operon leader peptide -
QR62_RS12395 2551941..2552192 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
QR62_RS12400 2552189..2552443 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
QR62_RS12405 2552526..2553350 + 825 WP_000754737.1 SDR family oxidoreductase -
QR62_RS12410 2553396..2554325 + 930 WP_000803351.1 LysR family transcriptional regulator -
QR62_RS25590 2554540..2554602 + 63 WP_010723108.1 membrane protein YoeI -
QR62_RS12415 2554592..2555950 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
QR62_RS12420 2556129..2557187 + 1059 WP_000492339.1 transport protein YeeE -
QR62_RS12425 2557201..2557428 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T57329 WP_000767829.1 NZ_CP012869:2552189-2552443 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T57329 NZ_CP012869:2552189-2552443 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT57329 WP_001259255.1 NZ_CP012869:2551941-2552192 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT57329 NZ_CP012869:2551941-2552192 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References