57262

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3235368..3236040 Replicon chromosome
Accession NZ_CP012831
Organism Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)


Gene name higB Uniprot ID A0A2K4FWL2
Locus tag AO356_RS14255 Protein ID WP_060740345.1
Coordinates 3235368..3235733 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8QJ57
Locus tag AO356_RS14260 Protein ID WP_025215812.1
Coordinates 3235726..3236040 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO356_RS14245 3232520..3233314 - 795 WP_060740343.1 carbon-nitrogen hydrolase family protein -
AO356_RS14250 3233327..3235009 - 1683 WP_060740344.1 NAD(P)/FAD-dependent oxidoreductase -
AO356_RS14255 3235368..3235733 + 366 WP_060740345.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AO356_RS14260 3235726..3236040 + 315 WP_025215812.1 XRE family transcriptional regulator Antitoxin
AO356_RS14265 3236166..3236627 - 462 WP_060743121.1 Lrp/AsnC family transcriptional regulator -
AO356_RS14270 3236663..3238990 - 2328 WP_081015470.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - pilT / pilG / pilH / pilJ 2997456..3236143 238687


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(5-86)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 14206.90 Da        Isoelectric Point: 5.6287

>T57262 WP_060740345.1 NZ_CP012831:3235368-3235733 [Pseudomonas fluorescens]
MKWDVEYTDEFERWWNRLCESEQDSVQASVMLLGDTGPYLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQGRWYQEYVPLAERLYDEHLEVLKREGFDNG

Download         Length: 366 bp

>T57262 NZ_CP012831:3235368-3235733 [Pseudomonas fluorescens]
ATGAAATGGGACGTTGAATACACTGACGAATTTGAGAGGTGGTGGAACCGTCTCTGTGAGTCAGAGCAAGACTCTGTACA
GGCCTCGGTAATGCTGCTCGGCGACACCGGGCCGTATCTGGGCTTTCCTCACACCAGTGATATCAAGGGTTCGCGCCATG
GCAACCTTCGTGAGTTACGAGTGCAACACGCGGGTAGGCCTTATCGTGTGCTCTATGCGTTTGATCCTCGCCGCTGCGCC
ATCCTGCTGATCGGGGGAGACAAGACCGGCCAGGGCCGTTGGTATCAGGAATATGTGCCTTTGGCCGAACGCCTCTATGA
CGAGCATTTGGAAGTATTGAAGAGAGAGGGCTTTGACAATGGCTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11872.62 Da        Isoelectric Point: 9.6213

>AT57262 WP_025215812.1 NZ_CP012831:3235726-3236040 [Pseudomonas fluorescens]
MAKKFSDLMARRSPDSQARAQALYQTLRAEMPLHELRQAQELSQEALAKALHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFSHPTP

Download         Length: 315 bp

>AT57262 NZ_CP012831:3235726-3236040 [Pseudomonas fluorescens]
ATGGCTAAGAAATTTTCCGACTTGATGGCGCGTCGGTCGCCTGACTCCCAGGCTCGTGCACAGGCTCTGTATCAGACGTT
GCGTGCAGAAATGCCGTTGCACGAGTTGCGCCAGGCGCAGGAGTTGAGCCAGGAGGCCTTGGCCAAGGCACTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAGCGTCGGACGGATATGTACATCAGCACGTTGCGAAATTACATCCGGGCCATGGGG
GGGGAATTGGAGATTATCGCGACCTTTCCAGAGGGGCAGGTCAGGATCGAAAATTTCTCCCATCCAACACCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4FWL2

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5FD61

References