Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 417548..418152 Replicon chromosome
Accession NZ_CP012831
Organism Pseudomonas fluorescens strain FW300-N2C3

Toxin (Protein)


Gene name higB Uniprot ID A0A2K4FHP6
Locus tag AO356_RS01870 Protein ID WP_060738343.1
Coordinates 417548..417862 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2K4FHV2
Locus tag AO356_RS01875 Protein ID WP_060738344.1
Coordinates 417865..418152 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AO356_RS01850 412843..413637 - 795 WP_060738339.1 exodeoxyribonuclease III -
AO356_RS01855 413740..415311 - 1572 WP_060738340.1 GGDEF domain-containing protein -
AO356_RS01860 415634..416992 + 1359 WP_060738341.1 chromate efflux transporter -
AO356_RS01865 416989..417420 - 432 WP_060738342.1 GNAT family N-acetyltransferase -
AO356_RS01870 417548..417862 + 315 WP_060738343.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AO356_RS01875 417865..418152 + 288 WP_060738344.1 helix-turn-helix domain-containing protein Antitoxin
AO356_RS01880 418295..418783 + 489 WP_060738345.1 hypothetical protein -
AO356_RS01885 418829..419065 - 237 WP_177344100.1 hypothetical protein -
AO356_RS01890 419210..420427 - 1218 WP_060738346.1 MFS transporter -
AO356_RS01895 420797..421705 + 909 WP_060738347.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(41-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(35-86)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11991.09 Da        Isoelectric Point: 10.3369

>T57259 WP_060738343.1 NZ_CP012831:417548-417862 [Pseudomonas fluorescens]
MIFIETPIFTRRVKELMDDDDFAVLQKIRVCNPSAGEVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKLIVEHWR

Download         Length: 315 bp

>T57259 NZ_CP012831:417548-417862 [Pseudomonas fluorescens]
ATGATTTTTATTGAAACTCCAATTTTTACCCGTCGGGTCAAAGAACTGATGGATGACGATGATTTCGCTGTCCTACAGAA
AATACGGGTCTGCAATCCTTCCGCTGGTGAAGTTATGGAAGCCACCGGGGGGATCCGAAAGATTCGTATCGCAGCCAAAG
GCCATGGCAAACGGGGGGGAGCCAGGGTGATCTACTACCACTTCGTCCATGCTTCGCGGATCGCGTTACTGATGATATAT
CCGAAAAATGAACAGCAAGACTTGACTGTCGATCAACGAAAAGCGTTGAAATTGATTGTCGAGCACTGGAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10683.36 Da        Isoelectric Point: 10.0864

>AT57259 WP_060738344.1 NZ_CP012831:417865-418152 [Pseudomonas fluorescens]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKAIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Download         Length: 288 bp

>AT57259 NZ_CP012831:417865-418152 [Pseudomonas fluorescens]
ATGAGCAAATTTTTCGATGAGCTGATGGAAAGCGTGCAGCAGATGGATGAAATCGTCCGAGGCGAACGCCAGCCATCACG
GGAATTCATCGTCGATGCACTGCAAGTGAAGGCGATACGCAAGGCTACCGGCCTGACCCAAGCCAAATTTGCAGCACTGA
TCGATGTCCAACTGGGCACGCTTCGCAACTGGGAGCAGGGACGGCGCGAGCCTACCGGCCCCGCTAAAGCGCTGCTGCGG
GCGATTCACAATGATCCGAAACACGTGATCAGCGCCTTAGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4FHP6

Antitoxin

Source ID Structure
AlphaFold DB A0A2K4FHV2

References