Detailed information of TA system TA057229

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2472629..2472815
• Replicon: chromosome
• Accession: NZ_CP012802
• Organism: Escherichia coli O157:H7 strain WS4202

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: AO055_RS12765
• Protein ID: WP_001459284.1
• Coordinates: 2472699..2472815 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2472629..2472687 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO055_RS28795	2468883..2468990	-	108	WP_001273654.1	hypothetical protein	-
AO055_RS12735	2469949..2470578	+	630	WP_000203825.1	anti-repressor protein	-
AO055_RS12740	2470626..2470847	+	222	WP_000763353.1	TraR/DksA family transcriptional regulator	-
AO055_RS12745	2470844..2471128	+	285	WP_001024844.1	DUF4752 family protein	-
AO055_RS31410	2471115..2471584	+	470	Protein_2416	ead/Ea22-like family protein	-
AO055_RS31415	2471606..2472013	+	408	WP_001120842.1	DUF551 domain-containing protein	-
AO055_RS12760	2472013..2472408	+	396	WP_000426668.1	hypothetical protein	-
-	2472629..2472687	-	59	-	-	Antitoxin
AO055_RS12765	2472699..2472815	+	117	WP_001459284.1	Hok/Gef family protein	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stx2B / stx2A	2467472..2532549	65077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-30)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4175.31 Da
• Isoelectric Point: 10.6248

>T57229 WP_001459284.1 NZ_CP012802:2472699-2472815 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALITKKDLCKGGIPRHL

Nucleotide

• Length: 117 bp

>T57229 NZ_CP012802:2472699-2472815 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGATAACGAAGAA
AGACCTCTGCAAGGGAGGAATCCCTCGCCACCTCTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT57229 NZ_CP012802:c2472687-2472629 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTTTGTGAAGCATAGATTGGGCCTCA

Structures

Antitoxin