Detailed information of TA system TA057226

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2009880..2010105
• Replicon: chromosome
• Accession: NZ_CP012802
• Organism: Escherichia coli O157:H7 strain WS4202

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: AO055_RS10090
• Protein ID: WP_000813258.1
• Coordinates: 2009880..2010035 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2010047..2010105 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AO055_RS10040	2004883..2005314	-	432	WP_001302123.1	tellurite resistance protein	-
AO055_RS10055	2005765..2006478	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
AO055_RS31245	2006614..2006811	-	198	WP_000917763.1	hypothetical protein	-
AO055_RS10065	2007036..2007590	-	555	WP_000640035.1	DUF1133 family protein	-
AO055_RS10070	2007653..2007958	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
AO055_RS10075	2007971..2009020	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
AO055_RS10080	2009022..2009294	-	273	WP_000191872.1	hypothetical protein	-
AO055_RS10085	2009416..2009760	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
AO055_RS10090	2009880..2010035	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2010047..2010105	+	59	-	-	Antitoxin
AO055_RS10095	2010326..2010883	-	558	WP_000104474.1	DUF551 domain-containing protein	-
AO055_RS10100	2010885..2011103	-	219	WP_000683609.1	DUF4014 family protein	-
AO055_RS10105	2011231..2011542	-	312	WP_001289673.1	hypothetical protein	-
AO055_RS10110	2011535..2011762	-	228	WP_000699809.1	hypothetical protein	-
AO055_RS10115	2011759..2012040	-	282	WP_000603384.1	DNA-binding protein	-
AO055_RS10120	2012073..2012789	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
AO055_RS31250	2012823..2013284	-	462	WP_000139447.1	replication protein	-
AO055_RS31255	2013277..2014320	-	1044	WP_001262402.1	hypothetical protein	-
AO055_RS10135	2014389..2014814	-	426	WP_000693878.1	Rha family transcriptional regulator	-
AO055_RS10140	2014798..2015040	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7' / paa	1972865..2073138	100273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T57226 WP_000813258.1 NZ_CP012802:c2010035-2009880 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T57226 NZ_CP012802:c2010035-2009880 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT57226 NZ_CP012802:2010047-2010105 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin