Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2261331..2261928 Replicon chromosome
Accession NZ_CP012753
Organism Klebsiella pneumoniae strain KP617

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AN966_RS11240 Protein ID WP_004142563.1
Coordinates 2261611..2261928 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AN966_RS11235 Protein ID WP_004142561.1
Coordinates 2261331..2261618 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN966_RS11205 2257411..2257659 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AN966_RS11210 2257677..2258018 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AN966_RS11215 2258049..2259164 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
AN966_RS11220 2259344..2259925 + 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
AN966_RS11225 2259925..2260293 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AN966_RS11230 2260413..2261063 + 651 Protein_2184 oxygen-insensitive NAD(P)H nitroreductase -
AN966_RS11235 2261331..2261618 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AN966_RS11240 2261611..2261928 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN966_RS11245 2262113..2263156 - 1044 WP_004178895.1 DUF2157 domain-containing protein -
AN966_RS11250 2263823..2264689 - 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
AN966_RS11255 2264798..2266225 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T57185 WP_004142563.1 NZ_CP012753:c2261928-2261611 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T57185 NZ_CP012753:c2261928-2261611 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT57185 WP_004142561.1 NZ_CP012753:c2261618-2261331 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT57185 NZ_CP012753:c2261618-2261331 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References