Detailed information of TA system TA057174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2476132..2476802
• Replicon: chromosome
• Accession: NZ_CP012750
• Organism: Glutamicibacter halophytocola strain KLBMP 5180

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: AOZ07_RS11385
• Protein ID: WP_060702102.1
• Coordinates: 2476443..2476802 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: AOZ07_RS11380
• Protein ID: WP_060703428.1
• Coordinates: 2476132..2476437 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOZ07_RS11355	2471551..2472978	+	1428	WP_194943653.1	MFS transporter	-
AOZ07_RS11360	2472995..2474167	+	1173	WP_084793226.1	amidohydrolase	-
AOZ07_RS11370	2475074..2475609	-	536	Protein_2227	isochorismatase family protein	-
AOZ07_RS11375	2475609..2475911	-	303	WP_060702101.1	HigA family addiction module antidote protein	-
AOZ07_RS11380	2476132..2476437	-	306	WP_060703428.1	helix-turn-helix domain-containing protein	Antitoxin
AOZ07_RS11385	2476443..2476802	-	360	WP_060702102.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOZ07_RS11390	2477294..2478025	+	732	WP_060702103.1	hypothetical protein	-
AOZ07_RS11395	2478286..2479206	+	921	WP_060702104.1	toll/interleukin-1 receptor domain-containing protein	-
AOZ07_RS18240	2479328..2479656	-	329	Protein_2233	Abi family protein	-
AOZ07_RS11400	2479653..2480351	-	699	WP_060702105.1	GNAT family N-acetyltransferase	-
AOZ07_RS11405	2480372..2480836	-	465	WP_207758452.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2468332..2515288	46956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-93)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13545.40 Da
• Isoelectric Point: 4.4991

>T57174 WP_060702102.1 NZ_CP012750:c2476802-2476443 [Glutamicibacter halophytocola]
MWNVDIELIELIEVWLAELDVDSREQVVAAIELLEDSGPQLGRPIVDTVKASRHKNIKELRPGSSGKSELRILFAFDPER
AAIMLIAGDKAGDWQGWYEKNLPHADRLFDEHLDKIRGE

Nucleotide

• Length: 360 bp

>T57174 NZ_CP012750:c2476802-2476443 [Glutamicibacter halophytocola]
TTGTGGAACGTGGACATCGAGCTCATCGAGCTCATCGAGGTTTGGCTTGCTGAGCTTGATGTTGATTCCCGGGAGCAAGT
GGTTGCCGCGATTGAACTGCTAGAGGATTCTGGGCCCCAGCTCGGACGACCCATTGTGGACACCGTAAAAGCATCTCGCC
ATAAGAATATAAAGGAACTTCGACCAGGTTCATCTGGCAAATCAGAATTGCGAATCTTGTTTGCGTTCGATCCGGAGCGG
GCGGCAATCATGCTAATTGCGGGCGATAAAGCTGGTGACTGGCAAGGCTGGTACGAGAAAAACCTTCCACATGCCGATCG
ATTGTTCGATGAACATTTAGACAAGATAAGAGGAGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11550.22 Da
• Isoelectric Point: 8.5142

>AT57174 WP_060703428.1 NZ_CP012750:c2476437-2476132 [Glutamicibacter halophytocola]
MAKSLKDFLAQNPVDRDRVEKHKQRMLAEVRAYRLRELREQAGLTQAELAERIGVGQRQVSKIEHGDLENAKIGTIRNYL
EAVGGELSLEYLMGDQRMQIA

Nucleotide

• Length: 306 bp

>AT57174 NZ_CP012750:c2476437-2476132 [Glutamicibacter halophytocola]
TTGGCCAAGTCTCTTAAGGACTTTCTCGCTCAGAATCCAGTCGATCGGGACCGGGTCGAGAAGCACAAGCAGAGAATGCT
TGCGGAGGTTCGTGCCTATCGCTTGCGCGAATTGCGTGAACAGGCTGGACTCACTCAAGCTGAGCTCGCTGAGCGCATTG
GTGTGGGGCAGCGTCAAGTCTCGAAGATTGAGCATGGAGATCTTGAGAATGCAAAAATCGGCACAATCCGCAACTATTTG
GAAGCTGTCGGAGGGGAGCTGTCCTTGGAATACCTGATGGGCGATCAGCGGATGCAGATAGCCTGA