Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3886489..3887086 Replicon chromosome
Accession NZ_CP012744
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH10

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AOG31_RS19500 Protein ID WP_004142563.1
Coordinates 3886769..3887086 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AOG31_RS19495 Protein ID WP_004142561.1
Coordinates 3886489..3886776 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOG31_RS19465 3882583..3882831 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AOG31_RS19470 3882849..3883190 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AOG31_RS19475 3883221..3884126 - 906 WP_062920869.1 MBL fold metallo-hydrolase -
AOG31_RS19480 3884514..3885083 + 570 WP_062920870.1 TetR/AcrR family transcriptional regulator -
AOG31_RS19485 3885083..3885451 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AOG31_RS19490 3885571..3886224 + 654 Protein_3787 oxygen-insensitive NAD(P)H nitroreductase -
AOG31_RS19495 3886489..3886776 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AOG31_RS19500 3886769..3887086 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOG31_RS19505 3887271..3888314 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
AOG31_RS19510 3888981..3889847 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
AOG31_RS19515 3889956..3891383 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T57145 WP_004142563.1 NZ_CP012744:c3887086-3886769 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T57145 NZ_CP012744:c3887086-3886769 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT57145 WP_004142561.1 NZ_CP012744:c3886776-3886489 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT57145 NZ_CP012744:c3886776-3886489 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References