Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3964803..3965400 Replicon chromosome
Accession NZ_CP012743
Organism Klebsiella pneumoniae subsp. pneumoniae strain TGH8

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag AOD72_RS19745 Protein ID WP_004142563.1
Coordinates 3965083..3965400 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag AOD72_RS19740 Protein ID WP_004142561.1
Coordinates 3964803..3965090 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOD72_RS19710 3960897..3961145 + 249 WP_002893037.1 DUF1158 domain-containing protein -
AOD72_RS19715 3961163..3961504 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
AOD72_RS19720 3961535..3962440 - 906 WP_062920869.1 MBL fold metallo-hydrolase -
AOD72_RS19725 3962828..3963397 + 570 WP_062920870.1 TetR/AcrR family transcriptional regulator -
AOD72_RS19730 3963397..3963765 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
AOD72_RS19735 3963885..3964538 + 654 Protein_3858 oxygen-insensitive NAD(P)H nitroreductase -
AOD72_RS19740 3964803..3965090 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
AOD72_RS19745 3965083..3965400 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOD72_RS19750 3965585..3966628 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
AOD72_RS19755 3967422..3968288 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
AOD72_RS19760 3968397..3969824 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T57129 WP_004142563.1 NZ_CP012743:c3965400-3965083 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T57129 NZ_CP012743:c3965400-3965083 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT57129 WP_004142561.1 NZ_CP012743:c3965090-3964803 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT57129 NZ_CP012743:c3965090-3964803 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References