Detailed information of TA system TA057106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4600691..4601535
• Replicon: chromosome
• Accession: NZ_CP012735
• Organism: Shigella flexneri 1a strain 0228

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: AOT98_RS25195
• Protein ID: WP_014532172.1
• Coordinates: 4601074..4601535 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: AOT98_RS25185
• Protein ID: WP_005053053.1
• Coordinates: 4600691..4601002 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOT98_RS25160 (4596162)	4596162..4596659	+	498	Protein_4515	COX aromatic rich motif-containing protein	-
AOT98_RS25165 (4596681)	4596681..4598672	+	1992	Protein_4516	cytochrome o ubiquinol oxidase subunit I	-
AOT98_RS25170 (4598662)	4598662..4599276	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
AOT98_RS25175 (4599276)	4599276..4599605	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
AOT98_RS25180 (4599617)	4599617..4600507	+	891	WP_000971346.1	heme o synthase	-
AOT98_RS25185 (4600691)	4600691..4601002	+	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
AOT98_RS25195 (4601074)	4601074..4601535	+	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
AOT98_RS25205 (4602431)	4602431..4604584	+	2154	WP_005060811.1	SEL1-like repeat protein	-
AOT98_RS25210 (4604637)	4604637..4606367	+	1731	WP_000645013.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.004	flank	IS/Tn	-	-	4601583..4602086	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T57106 WP_014532172.1 NZ_CP012735:4601074-4601535 [Shigella flexneri 1a]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T57106 NZ_CP012735:4601074-4601535 [Shigella flexneri 1a]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT57106 WP_005053053.1 NZ_CP012735:4600691-4601002 [Shigella flexneri 1a]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT57106 NZ_CP012735:4600691-4601002 [Shigella flexneri 1a]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0