Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4563735..4564353 Replicon chromosome
Accession NZ_CP012735
Organism Shigella flexneri 1a strain 0228

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOT98_RS24995 Protein ID WP_001291435.1
Coordinates 4564135..4564353 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag AOT98_RS24990 Protein ID WP_000344797.1
Coordinates 4563735..4564109 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT98_RS24980 (4558824) 4558824..4560017 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOT98_RS24985 (4560040) 4560040..4563189 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
AOT98_RS24990 (4563735) 4563735..4564109 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
AOT98_RS24995 (4564135) 4564135..4564353 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOT98_RS25000 (4564525) 4564525..4565076 + 552 Protein_4484 maltose O-acetyltransferase -
AOT98_RS25005 (4565192) 4565192..4565662 + 471 WP_000136192.1 YlaC family protein -
AOT98_RS25010 (4565826) 4565826..4567376 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOT98_RS25015 (4567418) 4567418..4567771 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOT98_RS25025 (4568150) 4568150..4568461 + 312 Protein_4488 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4568491..4569819 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T57105 WP_001291435.1 NZ_CP012735:4564135-4564353 [Shigella flexneri 1a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T57105 NZ_CP012735:4564135-4564353 [Shigella flexneri 1a]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT57105 WP_000344797.1 NZ_CP012735:4563735-4564109 [Shigella flexneri 1a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT57105 NZ_CP012735:4563735-4564109 [Shigella flexneri 1a]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References