Detailed information of TA system TA057094

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3708025..3708250
• Replicon: chromosome
• Accession: NZ_CP012735
• Organism: Shigella flexneri 1a strain 0228

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: AOT98_RS20280
• Protein ID: WP_000813254.1
• Coordinates: 3708095..3708250 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3708025..3708083 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOT98_RS30390	3704023..3704192	+	170	Protein_3600	hypothetical protein	-
AOT98_RS20240	3704338..3705084	+	747	WP_000788996.1	ATP-binding protein	-
AOT98_RS20245	3705099..3705521	+	423	WP_001118168.1	DUF977 family protein	-
AOT98_RS20250	3705579..3705935	+	357	WP_005048249.1	hypothetical protein	-
AOT98_RS20255	3706028..3706246	+	219	WP_000256998.1	DUF4014 family protein	-
AOT98_RS20260	3706248..3706613	+	366	WP_001229297.1	HNH endonuclease signature motif containing protein	-
AOT98_RS20265	3706610..3707275	+	666	WP_000208062.1	hypothetical protein	-
AOT98_RS20270	3707275..3707640	+	366	WP_000610655.1	DUF551 domain-containing protein	-
-	3708025..3708083	-	59	-	-	Antitoxin
AOT98_RS20280	3708095..3708250	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
AOT98_RS20295	3709587..3710186	+	600	WP_000940329.1	DUF1367 family protein	-
AOT98_RS20300	3710186..3710476	+	291	WP_000228038.1	DUF1364 domain-containing protein	-
AOT98_RS20305	3710473..3711027	+	555	Protein_3612	DUF1133 family protein	-
AOT98_RS20310	3711180..3711353	+	174	WP_000504450.1	hypothetical protein	-
AOT98_RS20315	3711415..3712554	+	1140	WP_000088354.1	IS3 family transposase	-
AOT98_RS20320	3712547..3713107	+	561	WP_072075205.1	ORF6N domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	3696012..3764878	68866

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T57094 WP_000813254.1 NZ_CP012735:3708095-3708250 [Shigella flexneri 1a]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T57094 NZ_CP012735:3708095-3708250 [Shigella flexneri 1a]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT57094 NZ_CP012735:c3708083-3708025 [Shigella flexneri 1a]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin