57086

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 14545..15239 Replicon chromosome
Accession NZ_CP012735
Organism Shigella flexneri 1a strain 0228

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag AOT98_RS00940 Protein ID WP_001263491.1
Coordinates 14545..14943 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag AOT98_RS00945 Protein ID WP_000554759.1
Coordinates 14946..15239 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT98_RS00915 (9885) 9885..10343 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
AOT98_RS00920 (10605) 10605..12061 + 1457 Protein_16 cytosol nonspecific dipeptidase -
AOT98_RS00925 (12118) 12118..12678 - 561 Protein_17 peptide chain release factor H -
AOT98_RS00930 (12794) 12794..13837 - 1044 WP_005053205.1 RNA ligase RtcB family protein -
AOT98_RS00935 (14083) 14083..14535 - 453 WP_001059860.1 GNAT family N-acetyltransferase -
AOT98_RS00940 (14545) 14545..14943 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AOT98_RS00945 (14946) 14946..15239 - 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AOT98_RS00950 (15291) 15291..16346 - 1056 WP_001226172.1 DNA polymerase IV -
AOT98_RS00955 (16417) 16417..17202 - 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
AOT98_RS00960 (17174) 17174..18886 + 1713 Protein_24 flagellar biosynthesis protein FlhA -
AOT98_RS00965 (19103) 19103..19600 - 498 WP_000006251.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T57086 WP_001263491.1 NZ_CP012735:c14943-14545 [Shigella flexneri 1a]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T57086 NZ_CP012735:c14943-14545 [Shigella flexneri 1a]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT57086 WP_000554759.1 NZ_CP012735:c15239-14946 [Shigella flexneri 1a]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT57086 NZ_CP012735:c15239-14946 [Shigella flexneri 1a]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References