Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4567245..4567780 Replicon chromosome
Accession NZ_CP012693
Organism Escherichia coli strain FORC_028

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag FORC28_RS23965 Protein ID WP_032183258.1
Coordinates 4567493..4567780 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FORC28_RS23960 Protein ID WP_000729704.1
Coordinates 4567245..4567505 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC28_RS23935 4562281..4563420 + 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -
FORC28_RS23940 4563404..4565497 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
FORC28_RS23945 4565602..4565880 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FORC28_RS23950 4565873..4566229 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FORC28_RS23955 4566286..4567059 - 774 WP_000543897.1 C40 family peptidase -
FORC28_RS23960 4567245..4567505 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FORC28_RS23965 4567493..4567780 + 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FORC28_RS23970 4567936..4568676 + 741 WP_001225679.1 murein L,D-transpeptidase -
FORC28_RS23975 4568647..4569414 - 768 WP_000333380.1 class II glutamine amidotransferase -
FORC28_RS23980 4569620..4570198 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T57031 WP_032183258.1 NZ_CP012693:4567493-4567780 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T57031 NZ_CP012693:4567493-4567780 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT57031 WP_000729704.1 NZ_CP012693:4567245-4567505 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT57031 NZ_CP012693:4567245-4567505 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References