Detailed information of TA system TA057016

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2295577..2295948
• Replicon: chromosome
• Accession: NZ_CP012693
• Organism: Escherichia coli strain FORC_028

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: FORC28_RS11585
• Protein ID: WP_042853000.1
• Coordinates: 2295754..2295948 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2295577..2295755 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC28_RS11545	2291019..2291675	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
FORC28_RS11550	2291777..2292007	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
FORC28_RS11555	2292146..2292520	+	375	WP_000204699.1	CopC domain-containing protein YobA	-
FORC28_RS11560	2292524..2293396	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
FORC28_RS11565	2293409..2293750	+	342	WP_000976492.1	YebY family protein	-
FORC28_RS11575	2294143..2295219	-	1077	WP_001189091.1	phage integrase Arm DNA-binding domain-containing protein	-
FORC28_RS30360	2295185..2295466	-	282	WP_001443927.1	excisionase	-
FORC28_RS11580	2295573..2295761	-	189	WP_001356607.1	DUF1187 family protein	-
-	2295577..2295755	+	179	NuclAT_0	-	Antitoxin
-	2295577..2295755	+	179	NuclAT_0	-	Antitoxin
-	2295577..2295755	+	179	NuclAT_0	-	Antitoxin
-	2295577..2295755	+	179	NuclAT_0	-	Antitoxin
FORC28_RS11585	2295754..2295948	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
FORC28_RS11590	2296005..2296814	-	810	WP_000166317.1	recombination protein RecT	-
FORC28_RS11595	2296807..2299458	-	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
FORC28_RS11600	2299557..2299832	-	276	WP_001307773.1	hypothetical protein	-
FORC28_RS11605	2299906..2300082	-	177	WP_000245534.1	hypothetical protein	-
FORC28_RS11610	2300076..2300297	-	222	WP_000560218.1	killing protein KilR	-
FORC28_RS11615	2300718..2300870	-	153	WP_001427316.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2280801..2339490	58689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T57016 WP_042853000.1 NZ_CP012693:c2295948-2295754 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T57016 NZ_CP012693:c2295948-2295754 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT57016 NZ_CP012693:2295577-2295755 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin